气候变化领域集成服务门户(CCPortal): Optimized CRISPR tools and site-directed transgenesis towards gene drive development in Culex quinquefasciatus mosquitoes

CCPortal

DOI	10.1038/s41467-021-23239-0
	Optimized CRISPR tools and site-directed transgenesis towards gene drive development in Culex quinquefasciatus mosquitoes
	Feng X.; López Del Amo V.; Mameli E.; Lee M.; Bishop A.L.; Perrimon N.; Gantz V.M.
发表日期	2021
ISSN	2041-1723
卷号	12 期号:1
英文摘要	Culex mosquitoes are a global vector for multiple human and animal diseases, including West Nile virus, lymphatic filariasis, and avian malaria, posing a constant threat to public health, livestock, companion animals, and endangered birds. While rising insecticide resistance has threatened the control of Culex mosquitoes, advances in CRISPR genome-editing tools have fostered the development of alternative genetic strategies such as gene drive systems to fight disease vectors. However, though gene-drive technology has quickly progressed in other mosquitoes, advances have been lacking in Culex. Here, we develop a Culex-specific Cas9/gRNA expression toolkit and use site-directed homology-based transgenesis to generate and validate a Culex quinquefasciatus Cas9-expressing line. We show that gRNA scaffold variants improve transgenesis efficiency in both Culex quinquefasciatus and Drosophila melanogaster and boost gene-drive performance in the fruit fly. These findings support future technology development to control Culex mosquitoes and provide valuable insight for improving these tools in other species. © 2021, The Author(s).
语种	英语
scopus关键词	guide RNA; guide RNA; disease vector; fly; gene expression; genome; mosquito; RNA; technological development; animal cell; animal experiment; Article; clustered regularly interspaced short palindromic repeat; controlled study; Culex quinquefasciatus; disease carrier; Drosophila melanogaster; embryo; gene drive technology; gene editing; gene expression; genetic variability; nonhuman; transgene; transgenics; animal; CRISPR Cas system; Culex; female; gene drive technology; genetics; insecticide resistance; male; mosquito control; mosquito vector; procedures; site directed mutagenesis; transgenic animal; Culex pipiens quinquefasciatus; Melanogaster; West Nile virus; Animals; Animals, Genetically Modified; CRISPR-Cas Systems; Culex; Drosophila melanogaster; Female; Gene Drive Technology; Insecticide Resistance; Male; Mosquito Control; Mosquito Vectors; Mutagenesis, Site-Directed; RNA, Guide
来源期刊	Nature Communications
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251442
作者单位	Section of Cell and Developmental Biology, University of California San Diego, La Jolla, CA, United States; Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, United States; Department of Microbiology, National Emerging Infectious Diseases Laboratories, Boston University, School of Medicine, Boston, MA, United States; HHMI, Harvard Medical School, Boston, MA, United States
推荐引用方式 GB/T 7714	Feng X.,López Del Amo V.,Mameli E.,et al. Optimized CRISPR tools and site-directed transgenesis towards gene drive development in Culex quinquefasciatus mosquitoes[J],2021,12(1).
APA	Feng X..,López Del Amo V..,Mameli E..,Lee M..,Bishop A.L..,...&Gantz V.M..(2021).Optimized CRISPR tools and site-directed transgenesis towards gene drive development in Culex quinquefasciatus mosquitoes.Nature Communications,12(1).
MLA	Feng X.,et al."Optimized CRISPR tools and site-directed transgenesis towards gene drive development in Culex quinquefasciatus mosquitoes".Nature Communications 12.1(2021).