气候变化领域集成服务门户(CCPortal): Genome-resolved metagenomics reveals role of iron metabolism in drought-induced rhizosphere microbiome dynamics

CCPortal

DOI	10.1038/s41467-021-23553-7
	Genome-resolved metagenomics reveals role of iron metabolism in drought-induced rhizosphere microbiome dynamics
	Xu L.; Dong Z.; Chiniquy D.; Pierroz G.; Deng S.; Gao C.; Diamond S.; Simmons T.; Wipf H.M.-L.; Caddell D.; Varoquaux N.; Madera M.A.; Hutmacher R.; Deutschbauer A.; Dahlberg J.A.; Guerinot M.L.; Purdom E.; Banfield J.F.; Taylor J.W.; Lemaux P.G.; Coleman-Derr D.
发表日期	2021
ISSN	2041-1723
卷号	12 期号:1
英文摘要	Recent studies have demonstrated that drought leads to dramatic, highly conserved shifts in the root microbiome. At present, the molecular mechanisms underlying these responses remain largely uncharacterized. Here we employ genome-resolved metagenomics and comparative genomics to demonstrate that carbohydrate and secondary metabolite transport functionalities are overrepresented within drought-enriched taxa. These data also reveal that bacterial iron transport and metabolism functionality is highly correlated with drought enrichment. Using time-series root RNA-Seq data, we demonstrate that iron homeostasis within the root is impacted by drought stress, and that loss of a plant phytosiderophore iron transporter impacts microbial community composition, leading to significant increases in the drought-enriched lineage, Actinobacteria. Finally, we show that exogenous application of iron disrupts the drought-induced enrichment of Actinobacteria, as well as their improvement in host phenotype during drought stress. Collectively, our findings implicate iron metabolism in the root microbiome’s response to drought and may inform efforts to improve plant drought tolerance to increase food security. © 2021, This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.
语种	英语
scopus关键词	carbohydrate; iron; RNA 16S; bacterium; drought; genome; genomics; homeostasis; metabolism; microbial activity; microbial community; rhizosphere; secondary metabolite; Actinobacteria; Article; bacterial gene; comparative genomics; controlled study; drought; drought stress; drought tolerance; experimental design; food security; functional status; gene expression; gene ontology; genome; iron homeostasis; iron metabolism; iron transport; maize; metagenome; metagenomics; microbial community; nonhuman; phenotype; photosynthesis; phylogenetic tree; plant growth; plant root; rhizosphere; rhizosphere microbiome; RNA sequencing; sorghum; Streptomyces; acclimatization; crop production; genetics; metabolism; metagenomics; microbiology; microflora; physiological stress; physiology; procedures; Actinobacteria; Bacteria (microorganisms); Acclimatization; Actinobacteria; Crop Production; Droughts; Food Security; Iron; Metagenomics; Microbiota; Plant Roots; Rhizosphere; RNA-Seq; Soil Microbiology; Sorghum; Stress, Physiological
来源期刊	Nature Communications
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251433
作者单位	Department of Plant and Microbial Biology, University of California, Berkeley, CA, United States; State Key Laboratory of Plant Physiology and Biochemistry, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing, China; Department of Energy, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States; Department of Earth and Planetary Science, University of California, Berkeley, CA, United States; Plant Gene Expression Center, USDA-ARS, Albany, CA, United States; CNRS, University Grenoble Alpes, TIMC-IMAG, Grenoble, France; Westside Research & Extension Center, UC Department of Plant Sciences, University of California, Davis, CA, United States; Kearney Agricultural Research & Extension Center, Parlier, CA, United States; Department of Biological Scienes, Dartmouth College, Hanover, NH, United States; Department of Statistics, University of California, Berkeley, CA, United States
推荐引用方式 GB/T 7714	Xu L.,Dong Z.,Chiniquy D.,et al. Genome-resolved metagenomics reveals role of iron metabolism in drought-induced rhizosphere microbiome dynamics[J],2021,12(1).
APA	Xu L..,Dong Z..,Chiniquy D..,Pierroz G..,Deng S..,...&Coleman-Derr D..(2021).Genome-resolved metagenomics reveals role of iron metabolism in drought-induced rhizosphere microbiome dynamics.Nature Communications,12(1).
MLA	Xu L.,et al."Genome-resolved metagenomics reveals role of iron metabolism in drought-induced rhizosphere microbiome dynamics".Nature Communications 12.1(2021).