气候变化领域集成服务门户(CCPortal): The origin and impeded dissemination of the DNA phosphorothioation system in prokaryotes

CCPortal

DOI	10.1038/s41467-021-26636-7
	The origin and impeded dissemination of the DNA phosphorothioation system in prokaryotes
	Jian H.; Xu G.; Yi Y.; Hao Y.; Wang Y.; Xiong L.; Wang S.; Liu S.; Meng C.; Wang J.; Zhang Y.; Chen C.; Feng X.; Luo H.; Zhang H.; Zhang X.; Wang L.; Wang Z.; Deng Z.; Xiao X.
发表日期	2021
ISSN	2041-1723
卷号	12 期号:1
英文摘要	Phosphorothioate (PT) modification by the dnd gene cluster is the first identified DNA backbone modification and constitute an epigenetic system with multiple functions, including antioxidant ability, restriction modification, and virus resistance. Despite these advantages for hosting dnd systems, they are surprisingly distributed sporadically among contemporary prokaryotic genomes. To address this ecological paradox, we systematically investigate the occurrence and phylogeny of dnd systems, and they are suggested to have originated in ancient Cyanobacteria after the Great Oxygenation Event. Interestingly, the occurrence of dnd systems and prophages is significantly negatively correlated. Further, we experimentally confirm that PT modification activates the filamentous phage SW1 by altering the binding affinity of repressor and the transcription level of its encoding gene. Competition assays, concurrent epigenomic and transcriptomic sequencing subsequently show that PT modification affects the expression of a variety of metabolic genes, which reduces the competitive fitness of the marine bacterium Shewanella piezotolerans WP3. Our findings strongly suggest that a series of negative effects on microorganisms caused by dnd systems limit horizontal gene transfer, thus leading to their sporadic distribution. Overall, our study reveals putative evolutionary scenario of the dnd system and provides novel insights into the physiological and ecological influences of PT modification. © 2021, The Author(s).
语种	英语
scopus关键词	antioxidant; bacterium; DNA; fitness; gene expression; phylogeny; Bacteria (microorganisms); Cyanobacteria; Prokaryota; Shewanella; DNA; transcriptome; genetics; metabolism; phylogeny; Shewanella; DNA; Phylogeny; Shewanella; Transcriptome
来源期刊	Nature Communications
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251315
作者单位	State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Development Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, China; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, China; Simon F. S. Li Marine Science Laboratory, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong; Grandomics Biosciences, Wuhan, China
推荐引用方式 GB/T 7714	Jian H.,Xu G.,Yi Y.,et al. The origin and impeded dissemination of the DNA phosphorothioation system in prokaryotes[J],2021,12(1).
APA	Jian H..,Xu G..,Yi Y..,Hao Y..,Wang Y..,...&Xiao X..(2021).The origin and impeded dissemination of the DNA phosphorothioation system in prokaryotes.Nature Communications,12(1).
MLA	Jian H.,et al."The origin and impeded dissemination of the DNA phosphorothioation system in prokaryotes".Nature Communications 12.1(2021).