气候变化领域集成服务门户(CCPortal): Cellular-resolution gene expression profiling in the neonatal marmoset brain reveals dynamic species- And region-specific differences

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DOI	10.1073/pnas.2020125118
	Cellular-resolution gene expression profiling in the neonatal marmoset brain reveals dynamic species- And region-specific differences
	Kita Y.; Nishibe H.; Wang Y.; Hashikawa T.; Kikuchi S.S.; Mami U.; Yoshida A.C.; Yoshida C.; Kawase T.; Ishii S.; Skibbe H.; Shimogori T.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:18
英文摘要	Precise spatiotemporal control of gene expression in the developing brain is critical for neural circuit formation, and comprehensive expression mapping in the developing primate brain is crucial to understand brain function in health and disease. Here, we developed an unbiased, automated, large-scale, cellular-resolution in situ hybridization (ISH)-based gene expression profiling system (GePS) and companion analysis to reveal gene expression patterns in the neonatal New World marmoset cortex, thalamus, and striatum that are distinct from those in mice. Gene-ontology analysis of marmoset-specific genes revealed associations with catalytic activity in the visual cortex and neuropsychiatric disorders in the thalamus. Cortically expressed genes with clear area boundaries were used in a three-dimensional cortical surface mapping algorithm to delineate higher-order cortical areas not evident in two-dimensional ISH data. GePS provides a powerful platform to elucidate the molecular mechanisms underlying primate neurobiology and developmental psychiatric and neurological disorders. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Database; Developmental disorder; Evolution; Gene expression; Nonhuman primate
语种	英语
scopus关键词	animal cell; animal experiment; animal model; animal tissue; Article; automation; brain cortex; brain mapping; brain region; Callitrichinae; catalysis; controlled study; corpus striatum; developmental disorder; gene; gene expression; gene expression profiling; gene identification; gene ontology; genomic in situ hybridization; geography; marmoset specific gene; mental disease; mouse; neurobiology; neurologic disease; newborn; nonhuman; primate; priority journal; region specific difference; species difference; thalamus; thalamus nucleus; three dimensional cortical surface mapping algorithm; three-dimensional imaging; two-dimensional imaging; visual cortex; animal; brain; Callithrix; gene expression regulation; genetics; growth, development and aging; human; in situ hybridization; metabolism; species difference; transcriptome; Animals; Animals, Newborn; Brain; Callithrix; Corpus Striatum; Gene Expression Regulation, Developmental; Humans; In Situ Hybridization; Mice; Species Specificity; Transcriptome; Visual Cortex
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251197
作者单位	Laboratory for Molecular Mechanisms of Brain Development, Center for Brain Science, RIKEN, Saitama, 351-0198, Japan; Integrated Systems Biology Laboratory, Department of Systems Science, Graduate School of Informatics, Kyoto University, Kyoto, 606-8501, Japan; Brain Image Analysis Unit, Center for Brain Science, RIKEN, Saitama, 351-0198, Japan
推荐引用方式 GB/T 7714	Kita Y.,Nishibe H.,Wang Y.,et al. Cellular-resolution gene expression profiling in the neonatal marmoset brain reveals dynamic species- And region-specific differences[J],2021,118(18).
APA	Kita Y..,Nishibe H..,Wang Y..,Hashikawa T..,Kikuchi S.S..,...&Shimogori T..(2021).Cellular-resolution gene expression profiling in the neonatal marmoset brain reveals dynamic species- And region-specific differences.Proceedings of the National Academy of Sciences of the United States of America,118(18).
MLA	Kita Y.,et al."Cellular-resolution gene expression profiling in the neonatal marmoset brain reveals dynamic species- And region-specific differences".Proceedings of the National Academy of Sciences of the United States of America 118.18(2021).