气候变化领域集成服务门户(CCPortal): Toll9 from Bombyx mori functions as a pattern recognition receptor that shares features with Toll-like receptor 4 from mammals

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DOI	10.1073/pnas.2103021118
	Toll9 from Bombyx mori functions as a pattern recognition receptor that shares features with Toll-like receptor 4 from mammals
	Zhang R.; Li X.; Zhang J.; Li Y.; Wang Y.; Song Y.; Ren F.; Yi H.; Deng X.; Zhong Y.; Cao Y.; Strand M.R.; Yu X.-Q.; Yang W.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:19
英文摘要	Toll/Toll-like receptors (TLRs) are key regulators of the innate immune system in both invertebrates and vertebrates. However, while mammalian TLRs directly recognize pathogen-associated molecular patterns, the insect Toll pathway is thought to be primarily activated by binding Spätzle cytokines that are processed from inactive precursors in response to microbial infection. Phylogenetic and structural data generated in this study supported earlier results showing that Toll9 members differ from other insect Tolls by clustering with the mammalian TLR4 group, which recognizes lipopolysaccharide (LPS) through interaction with myeloid differentiation-2 (MD-2)-like proteins. Functional experiments showed that BmToll9 from the silkmoth Bombyx mori also recognized LPS through interaction with two MD-2-like proteins, previously named BmEsr16 and BmPP, that we refer to in this study as BmMD-2A and BmMD-2B, respectively. A chimeric BmToll9-TLR4 receptor consisting of the BmToll9 ectodomain and mouse TLR4 transmembrane and Toll/ interleukin-1 (TIR) domains also activated LPS-induced release of inflammatory factors in murine cells but only in the presence of BmMD-2A or BmMD-2B. Overall, our results indicate that BmToll9 is a pattern recognition receptor for LPS that shares conserved features with the mammalian TLR4-MD-2-LPS pathway. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Immunity; Lipopolysaccharide; MD-2; ML proteins; Toll
语种	英语
scopus关键词	amino acid; cytokine; flagellin; immunoglobulin G; leucine rich repeat kinase 2; lipopolysaccharide; peptidoglycan; toll like receptor 4; toll like receptor 9; insect protein; lipopolysaccharide; pattern recognition receptor; polypeptide antibiotic agent; protein binding; toll like receptor 4; toll like receptor 9; amino acid sequence; animal experiment; antimicrobial activity; Article; Bombyx mori; chimera; controlled study; immunofluorescence assay; immunoprecipitation; innate immunity; mouse; nonhuman; pattern recognition; phylogeny; protein function; protein motif; protein protein interaction; receptor binding; animal; blood cell; body fat; Bombyx; C57BL mouse; cell line; cytology; drug effect; gene expression regulation; genetics; human; knockout mouse; mammal; metabolism; RAW 264.7 cell line; Animals; Antimicrobial Peptides; Bombyx; Cell Line; Fat Body; Gene Expression Regulation; Hemocytes; Humans; Insect Proteins; Lipopolysaccharides; Mammals; Mice; Mice, Inbred C57BL; Mice, Knockout; Protein Binding; RAW 264.7 Cells; Receptors, Pattern Recognition; Toll-Like Receptor 4; Toll-Like Receptor 9
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251187
作者单位	Guangdong Laboratory for Lingnan Modern Agriculture, Laboratory of Insect Molecular Biology and Biotechnology, Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China; Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou, 510631, China; State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China; Biological Science Research Center, Southwest University, Chongqing, 400715, China; Department of Entomology, University of Georgia, Athens, GA 30602, United States
推荐引用方式 GB/T 7714	Zhang R.,Li X.,Zhang J.,et al. Toll9 from Bombyx mori functions as a pattern recognition receptor that shares features with Toll-like receptor 4 from mammals[J],2021,118(19).
APA	Zhang R..,Li X..,Zhang J..,Li Y..,Wang Y..,...&Yang W..(2021).Toll9 from Bombyx mori functions as a pattern recognition receptor that shares features with Toll-like receptor 4 from mammals.Proceedings of the National Academy of Sciences of the United States of America,118(19).
MLA	Zhang R.,et al."Toll9 from Bombyx mori functions as a pattern recognition receptor that shares features with Toll-like receptor 4 from mammals".Proceedings of the National Academy of Sciences of the United States of America 118.19(2021).