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DOI	10.1073/pnas.2022599118
	Presynaptic coupling by electrical synapses coordinates a rhythmic behavior by synchronizing the activities of a neuron pair
	Choi U.; Wang H.; Hu M.; Kim S.; Sieburth D.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:20
英文摘要	Electrical synapses are specialized structures that mediate the flow of electrical currents between neurons and have well known roles in synchronizing the activities of neuronal populations, both by mediating the current transfer from more active to less active neurons and by shunting currents from active neurons to their less active neighbors. However, how these positive and negative functions of electrical synapses are coordinated to shape rhythmic synaptic outputs and behavior is not well understood. Here, using a combination of genetics, behavioral analysis, and live calcium imaging in Caenorhabditis elegans, we show that electrical synapses formed by the gap junction protein INX-1/innexin couple the presynaptic terminals of a pair of motor neurons (AVL and DVB) to synchronize their activation in response to a pacemaker signal. Live calcium imaging reveals that inx-1/innexin mutations lead to asynchronous activation of AVL and DVB, due, in part, to loss of AVL-mediated activation of DVB by the pacemaker. In addition, loss of inx-1 leads to the ectopic activation of DVB at inappropriate times during the cycle through the activation of the L-type voltage-gated calcium channel EGL-19. We propose that electrical synapses between AVL and DVB presynaptic terminals function to ensure the precise and robust execution of a specific step in a rhythmic behavior by both synchronizing the activities of presynaptic terminals in response to pacemaker signaling and by inhibiting their activation in between cycles when pacemaker signaling is low. © 2021 National Academy of Sciences. All rights reserved.
语种	英语
scopus关键词	calcium channel affecting agent; egl 19; gap junction protein; innexin 1; unclassified drug; Caenorhabditis elegans protein; calcium; calcium channel; Egl-19 protein, C elegans; gap junction protein; green fluorescent protein; muscle protein; photoprotein; red fluorescent protein; 4 aminobutyric acid release; animal cell; Article; Caenorhabditis elegans; calcium transport; cell synchronization; controlled study; electrical synapse; live calcium imaging; microscopy; motoneuron; muscle contraction; mutation; nerve ending; neuromuscular function; nonhuman; protein function; animal; cytology; electrical synapse; gap junction; gene expression regulation; genetics; metabolism; molecular imaging; motoneuron; periodicity; reporter gene; synaptic transmission; ultrastructure; Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Calcium; Calcium Channels; Connexins; Electrical Synapses; Gap Junctions; Gene Expression Regulation; Genes, Reporter; Green Fluorescent Proteins; Luminescent Proteins; Molecular Imaging; Motor Neurons; Muscle Proteins; Periodicity; Presynaptic Terminals; Synaptic Transmission
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251186
作者单位	Development, Stem Cell, and Regenerative Medicine Graduate Program, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, United States; Zilkha Neurogenetic Institute, University of Southern California, Los Angeles, CA 90033, United States; Department of Physiology and Neuroscience, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, United States
推荐引用方式 GB/T 7714	Choi U.,Wang H.,Hu M.,et al. Presynaptic coupling by electrical synapses coordinates a rhythmic behavior by synchronizing the activities of a neuron pair[J],2021,118(20).
APA	Choi U.,Wang H.,Hu M.,Kim S.,&Sieburth D..(2021).Presynaptic coupling by electrical synapses coordinates a rhythmic behavior by synchronizing the activities of a neuron pair.Proceedings of the National Academy of Sciences of the United States of America,118(20).
MLA	Choi U.,et al."Presynaptic coupling by electrical synapses coordinates a rhythmic behavior by synchronizing the activities of a neuron pair".Proceedings of the National Academy of Sciences of the United States of America 118.20(2021).