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DOI	10.1073/pnas.2021053118
	HBD1 protein with a tandem repeat of two HMG-box domains is a DNA clip to organize chloroplast nucleoids in Chlamydomonas reinhardtii
	Takusagawa M.; Kobayashi Y.; Fukao Y.; Hidaka K.; Endo M.; Sugiyama H.; Hamaji T.; Kato Y.; Miyakawa I.; Misumi O.; Shikanai T.; Nishimura Y.
发表日期	2021
ISSN	1091-6490
卷号	118 期号:20
英文摘要	Compaction of bulky DNA is a universal issue for all DNA-based life forms. Chloroplast nucleoids (chloroplast DNA-protein complexes) are critical for chloroplast DNA maintenance and transcription, thereby supporting photosynthesis, but their detailed structure remains enigmatic. Our proteomic analysis of chloroplast nucleoids of the green alga Chlamydomonas reinhardtii identified a protein (HBD1) with a tandem repeat of two DNA-binding high mobility group box (HMG-box) domains, which is structurally similar to major mitochondrial nucleoid proteins transcription factor A, mitochondrial (TFAM), and ARS binding factor 2 protein (Abf2p). Disruption of the HBD1 gene by CRISPR-Cas9-mediated genome editing resulted in the scattering of chloroplast nucleoids. This phenotype was complemented when intact HBD1 was reintroduced, whereas a truncated HBD1 with a single HMG-box domain failed to complement the phenotype. Furthermore, ectopic expression of HBD1 in the mitochondria of yeast Δabf2 mutant successfully complemented the defects, suggesting functional similarity between HBD1 and Abf2p. Furthermore, in vitro assays of HBD1, including the electrophoretic mobility shift assay and DNA origami/atomic force microscopy, showed that HBD1 is capable of introducing U-turns and cross-strand bridges, indicating that proteins with two HMG-box domains would function as DNA clips to compact DNA in both chloroplast and mitochondrial nucleoids. Copyright © 2021 the Author(s). Published by PNAS.
英文关键词	chloroplast nucleoid; HMG-box domain; mitochondrial nucleoid
语种	英语
scopus关键词	chloroplast DNA; chloroplast protein; protein binding; Chlamydomonas reinhardtii; chloroplast genome; classification; gene expression regulation; genetics; high mobility group box domain; mass spectrometry; metabolism; mutation; phylogeny; procedures; proteomics; tandem repeat; Chlamydomonas reinhardtii; Chloroplast Proteins; DNA, Chloroplast; Gene Expression Regulation; Genome, Chloroplast; HMG-Box Domains; Mass Spectrometry; Mutation; Phylogeny; Protein Binding; Proteomics; Tandem Repeat Sequences
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251184
作者单位	Department of Botany, Graduate School of Science, Kyoto University, Kita-Shirakawa Oiwake-cho ,Sakyo-kuKyoto 606-8502, Japan; Graduate School of Science and Engineering, Ibaraki University, Bunkyo, Mito, Ibaraki 310-8512, Japan; Plant Global Educational Project, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan; Graduate School of Life Science, Ritsumeikan University, Kusatsu, 525-8577, Japan; Department of Chemistry, Graduate School of Science, Kyoto University, Kita-Shirakawa Oiwake-cho ,Sakyo-kuKyoto 606-8502, Japan; Institute for Integrated Cell-Material Sciences, Kyoto University, Sakyo-kuKyoto 606-8501, Japan; Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-kuTokyo 113-8657, Japan; Department of Biology, Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yoshida, Yamaguchi 753-8512, Japan; Department of Botany, Graduate School of Science, Kyoto University, Kita-Shirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
推荐引用方式 GB/T 7714	Takusagawa M.,Kobayashi Y.,Fukao Y.,et al. HBD1 protein with a tandem repeat of two HMG-box domains is a DNA clip to organize chloroplast nucleoids in Chlamydomonas reinhardtii[J],2021,118(20).
APA	Takusagawa M..,Kobayashi Y..,Fukao Y..,Hidaka K..,Endo M..,...&Nishimura Y..(2021).HBD1 protein with a tandem repeat of two HMG-box domains is a DNA clip to organize chloroplast nucleoids in Chlamydomonas reinhardtii.Proceedings of the National Academy of Sciences of the United States of America,118(20).
MLA	Takusagawa M.,et al."HBD1 protein with a tandem repeat of two HMG-box domains is a DNA clip to organize chloroplast nucleoids in Chlamydomonas reinhardtii".Proceedings of the National Academy of Sciences of the United States of America 118.20(2021).