气候变化领域集成服务门户(CCPortal): Knl1 participates in spindle assembly checkpoint signaling in maize

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DOI	10.1073/pnas.2022357118
	Knl1 participates in spindle assembly checkpoint signaling in maize
	Su H.; Liu Y.; Wang C.; Liu Y.; Feng C.; Sun Y.; Yuan J.; Birchler J.A.; Han F.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:20
英文摘要	The Knl1-Mis12-Ndc80 (KMN) network is an essential component of the kinetochore-microtubule attachment interface, which is required for genomic stability in eukaryotes. However, little is known about plant Knl1 proteins because of their complex evolutionary history. Here, we cloned the Knl1 homolog from maize (Zea mays) and confirmed it as a constitutive central kinetochore component. Functional assays demonstrated their conserved role in chromosomal congression and segregation during nuclear division, thus causing defective cell division during kernel development when Knl1 transcript was depleted. A 145 aa region in the middle of maize Knl1, that did not involve the MELT repeats, was associated with the interaction of spindle assembly checkpoint (SAC) components Bub1/Mad3 family proteins 1 and 2 (Bmf1/2) but not with the Bmf3 protein. They may form a helical conformation with a hydrophobic interface with the TPR domain of Bmf1/2, which is similar to that of vertebrates. However, this region detected in monocots shows extensive divergence in eudicots, suggesting that distinct modes of the SAC to kinetochore connection are present within plant lineages. These findings elucidate the conserved role of the KMN network in cell division and a striking dynamic of evolutionary patterns in the SAC signaling and kinetochore network. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cell division; Kinetochore; Knl1; Maize; SAC
语种	英语
scopus关键词	article; chromosome; controlled study; eudicot; fruit development; genetic transcription; hydrophobicity; kinetochore; M phase cell cycle checkpoint; maize; nonhuman; nuclear division; protein conformation; signal transduction; tetratricopeptide repeat; vertebrate; amino acid sequence; binding site; cell cycle checkpoint; chromosome segregation; classification; gene expression profiling; gene expression regulation; genetics; kinetochore; maize; metabolism; mutation; phylogeny; plant seed; procedures; sequence homology; signal transduction; spindle apparatus; transgenic plant; microtubule associated protein; plant protein; protein binding; Amino Acid Sequence; Binding Sites; Cell Cycle Checkpoints; Chromosome Segregation; Gene Expression Profiling; Gene Expression Regulation, Plant; Kinetochores; Microtubule-Associated Proteins; Mutation; Phylogeny; Plant Proteins; Plants, Genetically Modified; Protein Binding; RNA-Seq; Seeds; Sequence Homology, Amino Acid; Signal Transduction; Spindle Apparatus; Zea mays
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251178
作者单位	State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China; College of Advanced Agricultural Sciences, University of the Chinese Academy of Sciences, Beijing, 100049, China; Division of Biological Sciences, University of Missouri, Columbia, MO 65211-7400, United States
推荐引用方式 GB/T 7714	Su H.,Liu Y.,Wang C.,et al. Knl1 participates in spindle assembly checkpoint signaling in maize[J],2021,118(20).
APA	Su H..,Liu Y..,Wang C..,Liu Y..,Feng C..,...&Han F..(2021).Knl1 participates in spindle assembly checkpoint signaling in maize.Proceedings of the National Academy of Sciences of the United States of America,118(20).
MLA	Su H.,et al."Knl1 participates in spindle assembly checkpoint signaling in maize".Proceedings of the National Academy of Sciences of the United States of America 118.20(2021).