气候变化领域集成服务门户(CCPortal): Transposon-mediated insertional mutagenesis unmasks recessive insecticide resistance in the aphid Myzus persicae

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DOI	10.1073/pnas.2100559118
	Transposon-mediated insertional mutagenesis unmasks recessive insecticide resistance in the aphid Myzus persicae
	Panini M.; Chiesa O.; Troczka B.J.; Mallott M.; Manicardi G.C.; Cassanelli S.; Cominelli F.; Hayward A.; Mazzoni E.; Bass C.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:23
英文摘要	The evolution of resistance to insecticides threatens the sustainable control of many of the world's most damaging insect crop pests and disease vectors. To effectively combat resistance, it is important to understand its underlying genetic architecture, including the type and number of genetic variants affecting resistance and their interactions with each other and the environment. While significant progress has been made in characterizing the individual genes or mutations leading to resistance, our understanding of how genetic variants interact to influence its phenotypic expression remains poor. Here, we uncover a mechanism of insecticide resistance resulting from transposon-mediated insertional mutagenesis of a genetically dominant but insecticide-susceptible allele that enables the adaptive potential of a previously unavailable recessive resistance allele to be unlocked. Specifically, we identify clones of the aphid pest Myzus persicae that carry a resistant allele of the essential voltage-gated sodium channel (VGSC) gene with the recessive M918T and L1014F resistance mutations, in combination with an allele lacking these mutations but carrying a Mutator-like element transposon insertion that disrupts the coding sequence of the VGSC. This results in the down-regulation of the dominant susceptible allele and monoallelic expression of the recessive resistant allele, rendering the clones resistant to the insecticide bifenthrin. These findings are a powerful example of how transposable elements can provide a source of evolutionary potential that can be revealed by environmental and genetic perturbation, with applied implications for the control of highly damaging insect pests. © This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).
英文关键词	Resistance; Transposon; Voltage-gated sodium channel
语种	英语
scopus关键词	bifenthrin; voltage gated sodium channel; allele; Article; clone; controlled study; female; gene mutation; insecticide resistance; insertional mutagenesis; Myzus persicae; nonhuman; recessive inheritance; transposon; allele; animal; aphid; gene expression regulation; genetics; molecular evolution; recessive gene; Alleles; Animals; Aphids; DNA Transposable Elements; Evolution, Molecular; Genes, Recessive; Insecticide Resistance; Mutagenesis, Insertional
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251161
作者单位	Department of Sustainable Crop Production, Section Sustainable Crop and Food Protection, Università Cattolica del Sacro Cuore, Piacenza, 29122, Italy; College of Life and Environmental Sciences BioSciences, University of Exeter, Cornwall, TR10 9FE, United Kingdom; Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Modena, 42122, Italy
推荐引用方式 GB/T 7714	Panini M.,Chiesa O.,Troczka B.J.,et al. Transposon-mediated insertional mutagenesis unmasks recessive insecticide resistance in the aphid Myzus persicae[J],2021,118(23).
APA	Panini M..,Chiesa O..,Troczka B.J..,Mallott M..,Manicardi G.C..,...&Bass C..(2021).Transposon-mediated insertional mutagenesis unmasks recessive insecticide resistance in the aphid Myzus persicae.Proceedings of the National Academy of Sciences of the United States of America,118(23).
MLA	Panini M.,et al."Transposon-mediated insertional mutagenesis unmasks recessive insecticide resistance in the aphid Myzus persicae".Proceedings of the National Academy of Sciences of the United States of America 118.23(2021).