气候变化领域集成服务门户(CCPortal): Hormone-dependent activation and repression of microRNAs by the ecdysone receptor in the dengue vector mosquito Aedes aegypti

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DOI	10.1073/pnas.2102417118
	Hormone-dependent activation and repression of microRNAs by the ecdysone receptor in the dengue vector mosquito Aedes aegypti
	He Y.-Z.; Aksoy E.; Ding Y.; Raikhel A.S.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:26
英文摘要	Female mosquitoes transmit numerous devastating human diseases because they require vertebrate blood meal for egg development. MicroRNAs (miRNAs) play critical roles across multiple reproductive processes in female Aedes aegypti mosquitoes. However, how miRNAs are controlled to coordinate their activity with the demands of mosquito reproduction remains largely unknown. We report that the ecdysone receptor (EcR)-mediated 20-hydroxyecdysone (20E) signaling regulates miRNA expression in female mosquitoes. EcR RNAinterference silencing linked to small RNA-sequencing analysis reveals that EcR not only activates but also represses miRNA expression in the female mosquito fat body, a functional analog of the vertebrate liver. EcR directly represses the expression of clustered miR-275 and miR-305 before blood feeding when the 20E titer is low, whereas it activates their expression in response to the increased 20E titer after a blood meal. Furthermore, we find that SMRTER, an insect analog of the vertebrate nuclear receptor corepressors SMRT and N-CoR, interacts with EcR in a 20E-sensitive manner and is required for EcR-mediated repression of miRNA expression in Ae. aegypti mosquitoes. In addition, we demonstrate that miR-275 and miR-305 directly target glutamate semialdehyde dehydrogenase and AAEL009899, respectively, to facilitate egg development. This study reveals a mechanism for how miRNAs are controlled by the 20E signaling pathway to coordinate their activity with the demands of mosquito reproduction. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Coactivator; Corepressor; Small RNA; Steroid hormone; Vector-borne disease
语种	英语
scopus关键词	agarose; amino acid; cell nucleus receptor; complementary DNA; cycloheximide; ecdysone receptor; ecdysterone; glutamate gamma semialdehyde dehydrogenase; histone deacetylase; luciferase; microRNA; nuclear receptor corepressor; rapamycin; Renilla luciferin 2 monooxygenase; ribosephosphate pyrophosphokinase; transcriptome; vitellogenin; corepressor protein; ecdysone receptor; ecdysterone; histone deacetylase; insect protein; microRNA; steroid receptor; transcriptome; 3' untranslated region; Aedes aegypti; Anopheles gambiae; Article; down regulation; Drosophila; expression vector; female; gene expression; gene overexpression; gene silencing; immunoprecipitation; luciferase assay; mosquito vector; mRNA expression level; nonhuman; Plasmodium; protein synthesis; reporter gene; reverse transcription polymerase chain reaction; RNA extraction; RNA interference; RNA sequencing; upregulation; Aedes; animal; body fat; dengue; drug effect; feeding behavior; gene expression regulation; genetic transcription; genetics; growth, development and aging; metabolism; open reading frame; ovum; parasitology; promoter region; transcription initiation site; Aedes; Animals; Co-Repressor Proteins; Dengue; Ecdysterone; Fat Body; Feeding Behavior; Female; Gene Expression Regulation; Histone Deacetylases; Insect Proteins; MicroRNAs; Mosquito Vectors; Open Reading Frames; Ovum; Promoter Regions, Genetic; Receptors, Steroid; Transcription Initiation Site; Transcription, Genetic; Transcriptome
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251145
作者单位	Department of Entomology, University of California, Riverside, CA 92521, United States; Institute of Integrative Genome Biology, University of California, Riverside, CA 92521, United States; Graduate Program in Genetics Genomics and Bioinformatics, University of California, Riverside, CA 92521, United States
推荐引用方式 GB/T 7714	He Y.-Z.,Aksoy E.,Ding Y.,et al. Hormone-dependent activation and repression of microRNAs by the ecdysone receptor in the dengue vector mosquito Aedes aegypti[J],2021,118(26).
APA	He Y.-Z.,Aksoy E.,Ding Y.,&Raikhel A.S..(2021).Hormone-dependent activation and repression of microRNAs by the ecdysone receptor in the dengue vector mosquito Aedes aegypti.Proceedings of the National Academy of Sciences of the United States of America,118(26).
MLA	He Y.-Z.,et al."Hormone-dependent activation and repression of microRNAs by the ecdysone receptor in the dengue vector mosquito Aedes aegypti".Proceedings of the National Academy of Sciences of the United States of America 118.26(2021).