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DOI	10.1073/pnas.2026152118
	Loss of function of a DMR6 ortholog in tomato confers broad-spectrum disease resistance
	de Toledo Thomazella D.P.; Seong K.; Mackelprang R.; Dahlbeck D.; Geng Y.; Gill U.S.; Qi T.; Pham J.; Giuseppe P.; Lee C.Y.; Ortega A.; Cho M.-J.; Hutton S.F.; Staskawicz B.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:27
英文摘要	Plant diseases are among the major causes of crop yield losses around the world. To confer disease resistance, conventional breeding relies on the deployment of single resistance (R) genes. However, this strategy has been easily overcome by constantly evolving pathogens. Disabling susceptibility (S) genes is a promising alternative to R genes in breeding programs, as it usually offers durable and broad-spectrum disease resistance. In Arabidopsis, the S gene DMR6 (AtDMR6) encodes an enzyme identified as a susceptibility factor to bacterial and oomycete pathogens. Here, we present a model-to-crop translational work in which we characterize two AtDMR6 orthologs in tomato, SlDMR6-1 and SlDMR6-2. We show that SlDMR6-1, but not SlDMR6-2, is up-regulated by pathogen infection. In agreement, Sldmr6-1 mutants display enhanced resistance against different classes of pathogens, such as bacteria, oomycete, and fungi. Notably, disease resistance correlates with increased salicylic acid (SA) levels and transcriptional activation of immune responses. Furthermore, we demonstrate that SlDMR6-1 and SlDMR6-2 display SA-5 hydroxylase activity, thus contributing to the elucidation of the enzymatic function of DMR6. We then propose that SlDMR6 duplication in tomato resulted in subsequent subfunctionalization, in which SlDMR6-2 specialized in balancing SA levels in flowers/fruits, while SlDMR6-1 conserved the ability to fine-tune SA levels during pathogen infection of the plant vegetative tissues. Overall, this work not only corroborates a mechanism underlying SA homeostasis in plants, but also presents a promising strategy for engineering broad-spectrum and durable disease resistance in crops. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	CRISPR/Cas9 technology; Crop engineering; Disease resistance; DMR6; Salicylic acid
语种	英语
scopus关键词	CRISPR associated endonuclease Cas9; salicylic acid; transcriptome; 2,5-dihydroxybenzoic acid; Arabidopsis protein; gentisic acid; plant protein; Arabidopsis; Article; bacterial growth; biosynthesis; breeding; controlled study; disease resistance; disease severity; enzyme active site; enzyme activity; gene expression; gene mutation; high performance liquid chromatography; histochemistry; molecular docking; nonhuman; Oomycetes; phylogeny; plant disease; plant growth; plant immunity; protein expression; protein phosphorylation; protein purification; RNA sequencing; sequence homology; tomato; translational research; upregulation; biocatalysis; disease resistance; gene expression regulation; genetics; growth, development and aging; immunology; metabolism; microbiology; mutation; physiology; sequence homology; tomato; transgenic plant; Xanthomonas; Arabidopsis Proteins; Biocatalysis; Disease Resistance; Gene Expression Regulation, Plant; Gentisates; Lycopersicon esculentum; Mutation; Phylogeny; Plant Diseases; Plant Immunity; Plant Proteins; Plants, Genetically Modified; Salicylic Acid; Sequence Homology, Amino Acid; Transcriptome; Up-Regulation; Xanthomonas
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251137
作者单位	Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, United States; Innovative Genomics Institute, University of California, Berkeley, CA 94704, United States; Department of Plant Pathology, North Dakota State University, Fargo, ND 58108, United States; School of Life Sciences, Tsinghua University, Beijing, 100084, China; Brazilian Biorenewables National Laboratory, Brazilian Center for Research in Energy and Materials, SP, Campinas, 13083-100, Brazil; Horticultural Sciences Department, University of Florida, Gulf Coast Research and Education Center, Wimauma, FL 33598, United States
推荐引用方式 GB/T 7714	de Toledo Thomazella D.P.,Seong K.,Mackelprang R.,et al. Loss of function of a DMR6 ortholog in tomato confers broad-spectrum disease resistance[J],2021,118(27).
APA	de Toledo Thomazella D.P..,Seong K..,Mackelprang R..,Dahlbeck D..,Geng Y..,...&Staskawicz B..(2021).Loss of function of a DMR6 ortholog in tomato confers broad-spectrum disease resistance.Proceedings of the National Academy of Sciences of the United States of America,118(27).
MLA	de Toledo Thomazella D.P.,et al."Loss of function of a DMR6 ortholog in tomato confers broad-spectrum disease resistance".Proceedings of the National Academy of Sciences of the United States of America 118.27(2021).