气候变化领域集成服务门户(CCPortal): Pain and itch processing by subpopulations of molecularly diverse spinal and trigeminal projection neurons

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DOI	10.1073/pnas.2105732118
	Pain and itch processing by subpopulations of molecularly diverse spinal and trigeminal projection neurons
	Wercberger R.; Braz J.M.; Weinrich J.A.; Basbaum A.I.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:28
英文摘要	A remarkable molecular and functional heterogeneity of the primary sensory neurons and dorsal horn interneurons transmits pain- and or itch-relevant information, but the molecular signature of the projection neurons that convey the messages to the brain is unclear. Here, using retro-TRAP (translating ribosome affinity purification) and RNA sequencing, we reveal extensive molecular diversity of spino- and trigeminoparabrachial projection neurons. Among the many genes identified, we highlight distinct subsets of Cck+-, Nptx2+-, Nmb+-, and Crh+-expressing projection neurons. By combining in situ hybridization of retrogradely labeled neurons with Fos-based assays, we also demonstrate significant functional heterogeneity, including both convergence and segregation of pain- and itch-provoking inputs into molecularly diverse subsets of NK1R- and non-NK1R-expressing projection neurons. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Dorsal horn; Itch; Pain; Projection neurons; RNA-seq
语种	英语
scopus关键词	protein fos; chloroquine; neurokinin 1 receptor; RNA; adult; animal cell; animal experiment; animal model; animal tissue; Article; bioinformatics; Cck gene; cell subpopulation; controlled study; Crh gene; female; gene; gene expression; genetic heterogeneity; genetic variability; immunohistochemistry; immunoprecipitation; in situ hybridization; male; mouse; Nmb gene; nonhuman; Nptx2 gene; pain; polymerase chain reaction; projection neuron; protein purification; pruritus; RNA purification; RNA sequencing; spinal cord dorsal horn; spinal cord nerve cell; stimulus response; translating ribosome affinity purification; trigeminal nerve; animal; C57BL mouse; complication; drug effect; gene expression regulation; genetics; isolation and purification; metabolism; nerve cell; pain; pathology; physical stimulation; pruritus; spinal cord; trigeminal nerve; Animals; Chloroquine; Female; Gene Expression Regulation; Male; Mice, Inbred C57BL; Neurons; Pain; Physical Stimulation; Pruritus; Receptors, Neurokinin-1; RNA; Spinal Cord; Spinal Cord Dorsal Horn; Trigeminal Nerve
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251130
作者单位	Department of Anatomy, University of California, San Francisco, CA 94158, United States
推荐引用方式 GB/T 7714	Wercberger R.,Braz J.M.,Weinrich J.A.,et al. Pain and itch processing by subpopulations of molecularly diverse spinal and trigeminal projection neurons[J],2021,118(28).
APA	Wercberger R.,Braz J.M.,Weinrich J.A.,&Basbaum A.I..(2021).Pain and itch processing by subpopulations of molecularly diverse spinal and trigeminal projection neurons.Proceedings of the National Academy of Sciences of the United States of America,118(28).
MLA	Wercberger R.,et al."Pain and itch processing by subpopulations of molecularly diverse spinal and trigeminal projection neurons".Proceedings of the National Academy of Sciences of the United States of America 118.28(2021).