气候变化领域集成服务门户(CCPortal): OsPDCD5 negatively regulates plant architecture and grain yield in rice

CCPortal

DOI	10.1073/pnas.2018799118
	OsPDCD5 negatively regulates plant architecture and grain yield in rice
	Dong S.; Dong X.; Han X.; Zhang F.; Zhu Y.; Xin X.; Wang Y.; Hu Y.; Yuan D.; Wang J.; Huang Z.; Niu F.; Hu Z.; Yan P.; Cao L.; He H.; Fu J.; Xin Y.; Tan Y.; Mao B.; Zhao B.; Yang J.; Yuan L.; Luo X.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:29
英文摘要	Plant architecture is an important agronomic trait that affects crop yield. Here, we report that a gene involved in programmed cell death, OsPDCD5, negatively regulates plant architecture and grain yield in rice. We used the CRISPR/Cas9 system to introduce loss-of-function mutations into OsPDCD5 in 11 rice cultivars. Targeted mutagenesis of OsPDCD5 enhanced grain yield and improved plant architecture by increasing plant height and optimizing panicle type and grain shape. Transcriptome analysis showed that OsPDCD5 knockout affected auxin biosynthesis, as well as the gibberellin and cytokinin biosynthesis and signaling pathways. OsPDCD5 interacted directly with OsAGAP, and OsAGAP positively regulated plant architecture and grain yield in rice. Collectively, these findings demonstrate that OsPDCD5 is a promising candidate gene for breeding super rice cultivars with increased yield potential and superior quality. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	OsPDCD5 \| grain yield \| plant architecture \| programmed cell death polar auxin transport
语种	英语
scopus关键词	amylose; auxin; cytokinin; gibberellin; apoptosis regulatory protein; cytokinin; gibberellin; indoleacetic acid derivative; plant protein; protein binding; agricultural parameters; alkali spreading; Article; bioassay; brown rice rate; cellular distribution; chalkiness; controlled study; CRISPR-CAS9 system; gel consistency; gene; gene expression; genetic analysis; grain shape; grain yield; GUS staining; head milled rice rate; histology; knockout gene; loss of function mutation; luciferase assay; milled rice rate; nonhuman; OsAGAP gene; OsPDCD5 gene; panicle type; photosynthesis; plant gene; plant height; plant structures; pull-down assay; real time polymerase chain reaction; rice; RNA sequencing; staining; transcriptomics; Western blotting; yeast two hybrid assay; food grain; gene expression regulation; genetics; growth, development and aging; metabolism; mutagenesis; Oryza; plant breeding; plant structures; signal transduction; transgenic plant; Apoptosis Regulatory Proteins; Cytokinins; Edible Grain; Gene Expression Regulation, Plant; Gibberellins; Indoleacetic Acids; Mutagenesis; Oryza; Plant Breeding; Plant Proteins; Plant Structures; Plants, Genetically Modified; Protein Binding; Signal Transduction
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251121
作者单位	State Key Laboratory of Genetic Engineering and Engineering Research Center of Gene Technology (Ministry of Education), School of Life Sciences, Fudan University, Shanghai, 200438, China; Advanced Institute of Natural Sciences, Beijing Normal University at Zhuhai, Zhuhai, 440400, China; Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100101, China; State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, 410125, China; Ministry of Education Key Laboratory of Crop Physiology, Ecology and Genetic Breeding College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, China; Institute of Crop Breeding and Cultivation, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China
推荐引用方式 GB/T 7714	Dong S.,Dong X.,Han X.,et al. OsPDCD5 negatively regulates plant architecture and grain yield in rice[J],2021,118(29).
APA	Dong S..,Dong X..,Han X..,Zhang F..,Zhu Y..,...&Luo X..(2021).OsPDCD5 negatively regulates plant architecture and grain yield in rice.Proceedings of the National Academy of Sciences of the United States of America,118(29).
MLA	Dong S.,et al."OsPDCD5 negatively regulates plant architecture and grain yield in rice".Proceedings of the National Academy of Sciences of the United States of America 118.29(2021).