气候变化领域集成服务门户(CCPortal): Juvenile hormone regulation of microRNAs is mediated by E75 in the Dengue vector mosquito Aedes aegypti

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DOI	10.1073/pnas.2102851118
	Juvenile hormone regulation of microRNAs is mediated by E75 in the Dengue vector mosquito Aedes aegypti
	Aksoy E.; Raikhel A.S.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:29
英文摘要	MicroRNAs (miRNAs) are small noncoding RNAs that play critical roles in controlling posttranscriptional gene regulation and have a profound effect on mosquito reproduction and metabolism. Juvenile hormone (JH) is critical for achieving reproductive competence in the main vector of human arboviral diseases, Aedes aegypti. We report a JH-mediated mechanism governing miRNA expression. Using a transcription factor screen with multiple primary miRNA (pri-miRNA) promoters, we identified that the Ecdysone-induced protein E75 (E75) isoform (E75-RD) induced miRNA gene promoter activity. E75 binding sites were determined in miRNA promoters by means of cell transfection assay. E75-RD was found to be up-regulated by JH, as shown by the JH application and RNA interference (RNAi) of the JH receptor Methoprene-tolerant (Met). Small RNA sequencing from RNAi of Met and E75 displayed an overlapping miRNA cohort, suggesting E75 to be an intermediate component within the JH hierarchical network controlling miRNAs. Further experiments confirmed that E75-RD positively regulates several miRNAs including miR-2940. Reducing miR-2940 resulted in the arrest of follicle development and number of eggs laid. Performing miRNA target predictions and RT-qPCR from antagomir Ant-2940-3p–treated fat body tissues identified the mRNA target Clumsy (AAEL002518). The molecular interaction between this gene target and miR-2940 was confirmed using an in vitro dual luciferase assay in Drosophila S2 cells and in Ae. aegypti Aag2 cell lines. Finally, we performed a phenotypic rescue experiment to demonstrate that miR-2940/Clumsy is responsible for the disruption in egg development. Collectively, these results established the role of JH-mediated E75-RD in regulation of miRNA gene expression during the mosquito reproductive cycle. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Small noncoding RNA \| hormone \| isoform
语种	英语
scopus关键词	antagomir; ecdysone; ecdysone induced protein E75; isoprotein; juvenile hormone; methoprene; microRNA; microRNA 2940; transcription factor; unclassified drug; DNA binding protein; insect protein; juvenile hormone; microRNA; adipose tissue; adult; Aedes aegypti; animal cell; animal experiment; animal tissue; Article; binding site; cohort analysis; controlled study; Drosophila; female; gene activity; genetic transfection; in vitro study; luciferase assay; molecular interaction; nonhuman; ovary cycle; ovary follicle development; phenotype; prediction; promoter region; protein analysis; real time polymerase chain reaction; RNA gene; RNA interference; RNA sequencing; S2 cell line; upregulation; Aedes; animal; dengue; gene expression regulation; genetics; growth, development and aging; human; male; metabolism; mosquito vector; ovum; Aedes; Animals; Dengue; DNA-Binding Proteins; Female; Gene Expression Regulation, Developmental; Humans; Insect Proteins; Juvenile Hormones; Male; MicroRNAs; Mosquito Vectors; Ovum
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251117
作者单位	Department of Entomology, University of California, Riverside, CA 92521, United States; Graduate Program in Genetics, Genomics, and Bioinformatics, University of California, Riverside, CA 92521, United States; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA 02115, United States; Institute of Integrative Genome Biology, University of California, Riverside, CA 92521, United States
推荐引用方式 GB/T 7714	Aksoy E.,Raikhel A.S.. Juvenile hormone regulation of microRNAs is mediated by E75 in the Dengue vector mosquito Aedes aegypti[J],2021,118(29).
APA	Aksoy E.,&Raikhel A.S..(2021).Juvenile hormone regulation of microRNAs is mediated by E75 in the Dengue vector mosquito Aedes aegypti.Proceedings of the National Academy of Sciences of the United States of America,118(29).
MLA	Aksoy E.,et al."Juvenile hormone regulation of microRNAs is mediated by E75 in the Dengue vector mosquito Aedes aegypti".Proceedings of the National Academy of Sciences of the United States of America 118.29(2021).