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DOI	10.1073/pnas.2023216118
	Effects of sex and aging on the immune cell landscape as assessed by single-cell transcriptomic analysis
	Huang Z.; Chen B.; Liu X.; Li H.; Xie L.; Gao Y.; Duan R.; Li Z.; Zhang J.; Zheng Y.; Su W.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:33
英文摘要	Sex and aging influence the human immune system, resulting in disparate responses to infection, autoimmunity, and cancer. However, the impact of sex and aging on the immune system is not yet fully elucidated. Using small conditional RNA sequencing, we found that females had a lower percentage of natural killer (NK) cells and a higher percentage of plasma cells in peripheral blood compared with males. Bioinformatics revealed that young females exhibited an overrepresentation of pathways that relate to T and B cell activation. Moreover, cell-cell communication analysis revealed evidence of increased activity of the BAFF/APRIL systems in females. Notably, aging increased the percentage of monocytes and reduced the percentage of naïve T cells in the blood and the number of differentially expressed genes between the sexes. Aged males expressed higher levels of inflammatory genes. Collectively, the results suggest that females have more plasma cells in the circulation and a stronger BAFF/APRIL system, which is consistent with a stronger adaptive immune response. In contrast, males have a higher percentage of NK cells in blood and a higher expression of certain proinflammatory genes. Overall, this work expands our knowledge of sex differences in the immune system in humans. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Aging; Cell-cell communication; Immune responses; Sex; Single-cell sequencing
语种	英语
scopus关键词	APRIL protein; B cell activating factor; cytokine; transcriptome; adaptive immunity; adult; aged; aging; Article; B lymphocyte; cell activation; cell communication; female; human; immune response; immunocompetent cell; male; natural killer cell; normal human; plasma cell; RNA sequencing; sex; sex difference; signal transduction; T lymphocyte; transcriptomics; aging; gene expression regulation; genetics; immunology; immunosenescence; metabolism; middle aged; physiology; sex factor; single cell analysis; young adult; Adult; Aged; Aging; Cell Communication; Cytokines; Female; Gene Expression Regulation; Humans; Immunosenescence; Male; Middle Aged; Sex Factors; Single-Cell Analysis; T-Lymphocytes; Transcriptome; Young Adult
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251063
作者单位	State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, 510060, China; Department of Clinical Research Center, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, 510060, China
推荐引用方式 GB/T 7714	Huang Z.,Chen B.,Liu X.,et al. Effects of sex and aging on the immune cell landscape as assessed by single-cell transcriptomic analysis[J],2021,118(33).
APA	Huang Z..,Chen B..,Liu X..,Li H..,Xie L..,...&Su W..(2021).Effects of sex and aging on the immune cell landscape as assessed by single-cell transcriptomic analysis.Proceedings of the National Academy of Sciences of the United States of America,118(33).
MLA	Huang Z.,et al."Effects of sex and aging on the immune cell landscape as assessed by single-cell transcriptomic analysis".Proceedings of the National Academy of Sciences of the United States of America 118.33(2021).