| DOI | 10.1073/pnas.2102486118
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| A tomato LATERAL ORGAN BOUNDARIES transcription factor, SlLOB1, predominantly regulates cell wall and softening components of ripening |
| Shi Y.; Vrebalov J.; Zheng H.; Xu Y.; Yin X.; Liu W.; Liu Z.; Sorensen I.; Su G.; Ma Q.; Evanich D.; Rose J.K.C.; Fei Z.; van Eck J.; Thannhauser T.; Chen K.; Giovannoni J.J.
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| 发表日期 | 2021
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| ISSN | 0027-8424
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| 卷号 | 118期号:33 |
| 英文摘要 | Fruit softening is a key component of the irreversible ripening program, contributing to the palatability necessary for frugivoremediated seed dispersal. The underlying textural changes are complex and result from cell wall remodeling and changes in both cell adhesion and turgor. While a number of transcription factors (TFs) that regulate ripening have been identified, these affect most canonical ripening-related physiological processes. Here, we show that a tomato fruit ripening-specific LATERAL ORGAN BOUNDRIES (LOB) TF, SlLOB1, up-regulates a suite of cell wall-associated genes during late maturation and ripening of locule and pericarp tissues. SlLOB1 repression in transgenic fruit impedes softening, while overexpression throughout the plant under the direction of the 35s promoter confers precocious induction of cell wall gene expression and premature softening. Transcript and protein levels of the wall-loosening protein EXPANSIN1 (EXP1) are strongly suppressed in SlLOB1 RNA interference lines, while EXP1 is induced in SlLOB1-overexpressing transgenic leaves and fruit. In contrast to the role of ethylene and previously characterized ripening TFs, which are comprehensive facilitators of ripening phenomena including softening, SlLOB1 participates in a regulatory subcircuit predominant to cell wall dynamics and softening. © 2021 National Academy of Sciences. All rights reserved. |
| 英文关键词 | Cell wall; Ripening; SlLOB1; Softening; Transcription factor |
| 语种 | 英语
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| scopus关键词 | transcription factor; transcription factor SlLOB1; unclassified drug; carotenoid; ethylene; ethylene derivative; plant protein; transcription factor; Article; cell wall; controlled study; fruit ripening; gene expression; gene repression; gene targeting; nonhuman; plant gene; plant leaf; RNA interference; tomato; transgenic plant; upregulation; cell wall; food storage; fruit; gene expression regulation; gene silencing; genetics; growth, development and aging; metabolism; physiology; Carotenoids; Cell Wall; Ethylenes; Food Storage; Fruit; Gene Expression Regulation, Plant; Gene Silencing; Lycopersicon esculentum; Plant Proteins; Transcription Factors |
| 来源期刊 | Proceedings of the National Academy of Sciences of the United States of America
(IF:9.58[JCR-2018],10.6[5-Year]) |
| 文献类型 | 期刊论文
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| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/251051
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| 作者单位 | Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853, United States; Laboratory of Fruit Quality Biology, Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Hangzhou, 310058, China; Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, United States; Section of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853, United States; US Department of Agriculture-Agricultural Research Service, Robert W. Holley Center for Agriculture and Health, Cornell University, Ithaca, NY 14853, United States
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推荐引用方式
GB/T 7714 |
Shi Y.,Vrebalov J.,Zheng H.,et al. A tomato LATERAL ORGAN BOUNDARIES transcription factor, SlLOB1, predominantly regulates cell wall and softening components of ripening[J],2021,118(33).
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| APA |
Shi Y..,Vrebalov J..,Zheng H..,Xu Y..,Yin X..,...&Giovannoni J.J..(2021).A tomato LATERAL ORGAN BOUNDARIES transcription factor, SlLOB1, predominantly regulates cell wall and softening components of ripening.Proceedings of the National Academy of Sciences of the United States of America,118(33).
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| MLA |
Shi Y.,et al."A tomato LATERAL ORGAN BOUNDARIES transcription factor, SlLOB1, predominantly regulates cell wall and softening components of ripening".Proceedings of the National Academy of Sciences of the United States of America 118.33(2021).
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