气候变化领域集成服务门户(CCPortal): A role for Sfrp2 in cardiomyogenesis in vivo

CCPortal

DOI	10.1073/pnas.2103676118
	A role for Sfrp2 in cardiomyogenesis in vivo
	Gomez J.A.; Payne A.; Pratt R.E.; Hodgkinson C.P.; Dzau V.J.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:33
英文摘要	Cardiomyogenesis, the process by which the body generates cardiomyocytes, is poorly understood. We have recently shown that Sfrp2 promotes cardiomyogenesis in vitro. The objective of this study was to determine if Sfrp2 would similarly promote cardiomyogenesis in vivo. To test this hypothesis, we tracked multipotent cKit(+) cells in response to Sfrp2 treatment. In control adult mice, multipotent cKit(+) cells typically differentiated into endothelial cells but not cardiomyocytes. In contrast, Sfrp2 switched the fate of these cells. Following Sfrp2 injection, multipotent cKit(+) cells differentiated solely into cardiomyocytes. Sfrp2-derived cardiomyocytes integrated into the myocardium and exhibited identical physiological properties to preexisting native cardiomyocytes. The ability of Sfrp2 to promote cardiomyogenesis was further supported by tracking EdU-labeled cells. In addition, Sfrp2 did not promote the formation of new cardiomyocytes when the cKit(+) cell population was selectively ablated in vivo using a diphtheria toxin receptor-diphtheria toxin model. Notably, Sfrp2-induced cardiomyogenesis was associated with significant functional improvements in a cardiac injury model. In summary, our study further demonstrates the importance of Sfrp2 in cardiomyogenesis. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cell differentiation; Cell lineage; Heart injuries/pathology; Myocytes; Wnt signaling pathway
语种	英语
scopus关键词	secreted frizzled related protein 2; calcium; membrane protein; Sfrp2 protein, mouse; adult; animal cell; Article; cardiac muscle cell; cell differentiation; cell fate; cell lineage; cell population; controlled study; endothelium cell; in vivo study; mouse; nonhuman; protein expression; animal; cardiac muscle cell; gene expression regulation; heart contraction; heart infarction; metabolism; physiology; transgenic mouse; Animals; Calcium; Cell Differentiation; Gene Expression Regulation; Membrane Proteins; Mice; Mice, Transgenic; Myocardial Contraction; Myocardial Infarction; Myocytes, Cardiac
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251049
作者单位	Mandel Center for Hypertension and Atherosclerosis, Duke University Medical Center, Durham, NC 27710, United States; Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, United States
推荐引用方式 GB/T 7714	Gomez J.A.,Payne A.,Pratt R.E.,et al. A role for Sfrp2 in cardiomyogenesis in vivo[J],2021,118(33).
APA	Gomez J.A.,Payne A.,Pratt R.E.,Hodgkinson C.P.,&Dzau V.J..(2021).A role for Sfrp2 in cardiomyogenesis in vivo.Proceedings of the National Academy of Sciences of the United States of America,118(33).
MLA	Gomez J.A.,et al."A role for Sfrp2 in cardiomyogenesis in vivo".Proceedings of the National Academy of Sciences of the United States of America 118.33(2021).