气候变化领域集成服务门户(CCPortal): The radish Ogura fertility restorer impedes translation elongation along its cognate CMS-causing mRNA

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DOI	10.1073/pnas.2105274118
	The radish Ogura fertility restorer impedes translation elongation along its cognate CMS-causing mRNA
	Wang C.; Lezhneva L.; Arnal N.; Quadrado M.; Mireau H.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:35
英文摘要	The control of messenger RNA (mRNA) translation has been increasingly recognized as a key regulatory step for gene control, but clear examples in eukaryotes are still scarce. Nucleo-cytoplasmic male sterilities (CMS) represent ideal genetic models to dissect genetic interactions between the mitochondria and the nucleus in plants. This trait is determined by specific mitochondrial genes and is associatedwith a pollen sterility phenotype that can be suppressed by nuclear genes known as restorer-of-fertility (Rf). In this study, we focused on the Ogura CMS system in rapeseed and showed that reversion to male sterility by the PPR-B fertility restorer (also called Rfo) occurs through a specific translation inhibition of the mitochondria-encoded CMScausing mRNA orf138. We also demonstrate that PPR-B binds within the coding sequence of orf138 and acts as a ribosome blocker to specifically impede translation elongation along the orf138 mRNA. Rfo is the first recognized fertility restorer shown to act this way. These observations will certainly facilitate the development of synthetic fertility restorers for CMS systems in which efficient natural Rfs are lacking. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cytoplasmic male sterility; Mitochondria; Pentatricopeptide repeat; Restorer-of-fertility; Translation
语种	英语
scopus关键词	messenger RNA; plant protein; cytoplasm; gene expression regulation; genetics; metabolism; physiology; plant breeding; plant infertility; procedures; protein synthesis; Raphanus; Cytoplasm; Gene Expression Regulation, Plant; Plant Breeding; Plant Infertility; Plant Proteins; Protein Biosynthesis; Raphanus; RNA, Messenger
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251036
作者单位	Institut Jean-Pierre Bourgin, Institut National de Recherche pour l'Agriculture, Alimentation et l'Environnement, AgroParisTech, Université Paris-Saclay, Versailles, 78000, France
推荐引用方式 GB/T 7714	Wang C.,Lezhneva L.,Arnal N.,et al. The radish Ogura fertility restorer impedes translation elongation along its cognate CMS-causing mRNA[J],2021,118(35).
APA	Wang C.,Lezhneva L.,Arnal N.,Quadrado M.,&Mireau H..(2021).The radish Ogura fertility restorer impedes translation elongation along its cognate CMS-causing mRNA.Proceedings of the National Academy of Sciences of the United States of America,118(35).
MLA	Wang C.,et al."The radish Ogura fertility restorer impedes translation elongation along its cognate CMS-causing mRNA".Proceedings of the National Academy of Sciences of the United States of America 118.35(2021).