气候变化领域集成服务门户(CCPortal): Histone H3K27 methylation-mediated repression of Hairy regulates insect developmental transition by modulating ecdysone biosynthesis

CCPortal

DOI	10.1073/pnas.2101442118
	Histone H3K27 methylation-mediated repression of Hairy regulates insect developmental transition by modulating ecdysone biosynthesis
	Yang Y.; Zhao T.; Li Z.; Qian W.; Peng J.; Wei L.; Yuan D.; Li Y.; Xia Q.; Cheng D.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:35
英文摘要	Insect development is cooperatively orchestrated by the steroid hormone ecdysone and juvenile hormone (JH). The polycomb repressive complex 2 (PRC2)-mediated histone H3K27 trimethylation (H3K27me3) epigenetically silences gene transcription and is essential for a range of biological processes, but the functions of H3K27 methylation in insect hormone action are poorly understood. Here, we demonstrate that H3K27 methylation-mediated repression of Hairy transcription in the larval prothoracic gland (PG) is required for ecdysone biosynthesis in Bombyx and Drosophila. H3K27me3 levels in the PG are dynamically increased during the last larval instar. H3K27me3 reduction induced by the down-regulation of PRC2 activity via inhibitor treatment in Bombyx or PG-specific knockdown of the PRC2 component Su(z)12 in Drosophila diminishes ecdysone biosynthesis and disturbs the larval-pupal transition. Mechanistically, H3K27 methylation targets the JH signal transducer Hairy to repress its transcription in the PG; PG-specific knockdown or overexpression of the Hairy gene disrupts ecdysone biosynthesis and developmental transition; and developmental defects caused by PG-specific Su(z)12 knockdown can be partially rescued by Hairy down-regulation. The application of JH mimic to the PG decreases both H3K27me3 levels and Su(z)12 expression. Altogether, our study reveals that PRC2-mediated H3K27 methylation at Hairy in the PG during the larval period is required for ecdysone biosynthesis and the larval-pupal transition and provides insights into epigenetic regulation of the crosstalk between JH and ecdysone during insect development. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Ecdysone biosynthesis; H3K27 methylation; Hairy; Juvenile hormone; Repression
语种	英语
scopus关键词	basic helix loop helix transcription factor; Drosophila protein; ecdysone; hairy protein, Drosophila; histone; insect protein; juvenile hormone; repressor protein; steroid; animal; biosynthesis; Bombyx; Drosophila; gene expression regulation; genetic epigenesis; genetics; larva; metabolism; methylation; pupa; signal transduction; Animals; Basic Helix-Loop-Helix Transcription Factors; Bombyx; Drosophila; Drosophila Proteins; Ecdysone; Epigenesis, Genetic; Gene Expression Regulation, Developmental; Histones; Insect Proteins; Juvenile Hormones; Larva; Methylation; Pupa; Repressor Proteins; Signal Transduction; Steroids
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251035
作者单位	State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Chongqing, 400715, China; Chongqing Key Laboratory of Sericultural Science, Southwest University, Chongqing, 400715, China; School of Life Sciences, Southwest University, Chongqing, 400715, China
推荐引用方式 GB/T 7714	Yang Y.,Zhao T.,Li Z.,et al. Histone H3K27 methylation-mediated repression of Hairy regulates insect developmental transition by modulating ecdysone biosynthesis[J],2021,118(35).
APA	Yang Y..,Zhao T..,Li Z..,Qian W..,Peng J..,...&Cheng D..(2021).Histone H3K27 methylation-mediated repression of Hairy regulates insect developmental transition by modulating ecdysone biosynthesis.Proceedings of the National Academy of Sciences of the United States of America,118(35).
MLA	Yang Y.,et al."Histone H3K27 methylation-mediated repression of Hairy regulates insect developmental transition by modulating ecdysone biosynthesis".Proceedings of the National Academy of Sciences of the United States of America 118.35(2021).