气候变化领域集成服务门户(CCPortal): TEM1 combinatorially binds to FLOWERING LOCUS T and recruits a Polycomb factor to repress the floral transition in Arabidopsis

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DOI	10.1073/pnas.2103895118
	TEM1 combinatorially binds to FLOWERING LOCUS T and recruits a Polycomb factor to repress the floral transition in Arabidopsis
	Hu H.; Tian S.; Xie G.; Liu R.; Wang N.; Li S.; He Y.; Du J.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:35
英文摘要	Arabidopsis TEMPRANILLO 1 (TEM1) is a transcriptional repressor that participates in multiple flowering pathways and negatively regulates the juvenile-to-adult transition and the flowering transition. To understand the molecular basis for the site-specific regulation of FLOWERING LOCUS T (FT) by TEM1, we determined the structures of the two plant-specific DNA-binding domains in TEM1, AP2 and B3, in complex with their target DNA sequences from the FT gene 5'- untranslated region (5'-UTR), revealing the molecular basis for TEM1 specificity for its DNA targets. In vitro binding assays revealed that the combination of the AP2 and B3 binding sites greatly enhanced the overall binding of TEM1 to the FT 5'-UTR, indicating TEM1 combinatorically recognizes the FT gene 5'-UTR. We further showed that TEM1 recruits the Polycomb repressive complex 2 (PRC2) to the FT 5'-UTR. The simultaneous binding of the TEM1 AP2 and B3 domains to FT is necessary for deposition of H3K27me3 at the FT 5'-UTR and for the flowering repressor function of TEM1. Overall, our data suggest that the combinatorial recognition of FT 5'-UTR by TEM1 ensures H3K27me3 deposition to precisely regulate the floral transition. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Combinatorial binding; DNA binding; FLOWERING LOCUS T; PRC2; TEMPRANILLO 1
语种	英语
scopus关键词	Arabidopsis protein; FT protein, Arabidopsis; polycomb group protein; TEM1 protein, Arabidopsis; transcription factor; Arabidopsis; flower; gene expression regulation; genetics; growth, development and aging; metabolism; photoperiodicity; Arabidopsis; Arabidopsis Proteins; Flowers; Gene Expression Regulation, Plant; Photoperiod; Polycomb-Group Proteins; Transcription Factors
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251034
作者单位	National Key Laboratory of Plant Molecular Genetics, Shanghai Center for Plant Stress Biology, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, 201602, China; Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Institute of Plant and Food Science, School of Life Sciences, Southern University of Science and Technology, Shenzhen, 518055, China; University of Chinese Academy of Sciences, Beijing, 100049, China; School of Advanced Agricultural Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China; Peking University, Institute of Advanced Agricultural Sciences, Shandong, Weifang, 261000, China
推荐引用方式 GB/T 7714	Hu H.,Tian S.,Xie G.,et al. TEM1 combinatorially binds to FLOWERING LOCUS T and recruits a Polycomb factor to repress the floral transition in Arabidopsis[J],2021,118(35).
APA	Hu H..,Tian S..,Xie G..,Liu R..,Wang N..,...&Du J..(2021).TEM1 combinatorially binds to FLOWERING LOCUS T and recruits a Polycomb factor to repress the floral transition in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America,118(35).
MLA	Hu H.,et al."TEM1 combinatorially binds to FLOWERING LOCUS T and recruits a Polycomb factor to repress the floral transition in Arabidopsis".Proceedings of the National Academy of Sciences of the United States of America 118.35(2021).