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DOI	10.1073/pnas.2107320118
	Pathway conversion enables a double-lock mechanism to maintain DNA methylation and genome stability
	He L.; Zhao C.; Zhang Q.; Zinta G.; Wang D.; Lozano-Durán R.; Zhu J.-K.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:35
英文摘要	The CMT2 and RNA-directed DNA methylation (RdDM) pathways have been proposed to separately maintain CHH methylation in specific regions of the Arabidopsis thaliana genome. Here, we show that dysfunction of the chromatin remodeler DDM1 causes hundreds of genomic regions to switch from CMT2 dependency to RdDM dependency in DNA methylation. These converted loci are enriched at the edge regions of long transposable elements (TEs). Furthermore, we found that dysfunction in both DDM1 and RdDM causes strong reactivation of TEs and a burst of TE transposition in the first generation of mutant plants, indicating that the DDM1 and RdDM pathways together are critical to maintaining TE repression and protecting genomic stability. Our findings reveal the existence of a pathway conversion-based backup mechanism to guarantee the maintenance of DNA methylation and genome integrity. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	DNA methylation; Epigenetic regulation; Genomic stability; Transposon
语种	英语
scopus关键词	controlled study; DNA methylation; gene locus; gene repression; genetic regulation; genomic instability; methylome; nonhuman; promoter region; Review; RNA sequencing; signal transduction; transposon; Arabidopsis; chemistry; chromatin assembly and disassembly; gene expression regulation; genetic epigenesis; genetics; growth, development and aging; plant genome; Arabidopsis protein; plant DNA; Arabidopsis; Arabidopsis Proteins; Chromatin Assembly and Disassembly; DNA Methylation; DNA Transposable Elements; DNA, Plant; Epigenesis, Genetic; Gene Expression Regulation, Plant; Genome, Plant; Genomic Instability
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251031
作者单位	Shanghai Center for Plant Stress Biology, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, 201602, China; Department of Clinical Science Intervention and Technology, Karolinska Institutet, Stockholm, 14186, Sweden
推荐引用方式 GB/T 7714	He L.,Zhao C.,Zhang Q.,et al. Pathway conversion enables a double-lock mechanism to maintain DNA methylation and genome stability[J],2021,118(35).
APA	He L..,Zhao C..,Zhang Q..,Zinta G..,Wang D..,...&Zhu J.-K..(2021).Pathway conversion enables a double-lock mechanism to maintain DNA methylation and genome stability.Proceedings of the National Academy of Sciences of the United States of America,118(35).
MLA	He L.,et al."Pathway conversion enables a double-lock mechanism to maintain DNA methylation and genome stability".Proceedings of the National Academy of Sciences of the United States of America 118.35(2021).