气候变化领域集成服务门户(CCPortal): RNA-bound PGC-1α controls gene expression in liquid-like nuclear condensates

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DOI	10.1073/pnas.2105951118
	RNA-bound PGC-1α controls gene expression in liquid-like nuclear condensates
	Pérez-Schindler J.; Kohl B.; Schneider-Heieck K.; Leuchtmann A.B.; Henríquez-Olguín C.; Adak V.; Maier G.; Delezie J.; Sakoparnig T.; Vargas-Fernández E.; Karrer-Cardel B.; Ritz D.; Schmidt A.; Hondele M.; Jensen T.E.; Hiller S.; Handschin C.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:36
英文摘要	Plasticity of cells, tissues, and organs is controlled by the coordinated transcription of biological programs. However, the mechanisms orchestrating such context-specific transcriptional networks mediated by the dynamic interplay of transcription factors and coregulators are poorly understood. The peroxisome proliferator–activated receptor γ coactivator 1α (PGC-1α) is a prototypical master regulator of adaptive transcription in various cell types. We now uncovered a central function of the C-terminal domain of PGC-1α to bind RNAs and assemble multiprotein complexes including proteins that control gene transcription and RNA processing. These interactions are important for PGC-1α recruitment to chromatin in transcriptionally active liquid-like nuclear condensates. Notably, such a compartmentalization of active transcription mediated by liquid–liquid phase separation was observed in mouse and human skeletal muscle, revealing a mechanism by which PGC-1α regulates complex transcriptional networks. These findings provide a broad conceptual framework for context-dependent transcriptional control of phenotypic adaptations in metabolically active tissues. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Chromatin; Gene transcription; Liquid–liquid phase separation; RNA-binding protein; Transcriptional coactivator
语种	英语
scopus关键词	peroxisome proliferator activated receptor gamma coactivator 1alpha; RNA polymerase II; peroxisome proliferator activated receptor gamma coactivator 1alpha; PPARGC1A protein, human; RNA; aerobic metabolism; animal cell; Article; carboxy terminal sequence; conceptual framework; controlled study; gene control; gene expression; gene interaction; genetic transcription; human; in vitro study; male; mouse; nonhuman; normal human; protein function; RNA binding; RNA processing; skeletal muscle; transcription regulation; animal; C57BL mouse; cell line; cell nucleus; chromatin; gene expression regulation; metabolism; physiology; protein domain; Animals; Cell Line; Cell Nucleus; Chromatin; Gene Expression Regulation; Humans; Male; Mice; Mice, Inbred C57BL; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; Protein Domains; Protein Interaction Domains and Motifs; RNA
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251028
作者单位	Biozentrum, University of Basel, Basel, 4056, Switzerland; Section of Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, 2100, Denmark
推荐引用方式 GB/T 7714	Pérez-Schindler J.,Kohl B.,Schneider-Heieck K.,et al. RNA-bound PGC-1α controls gene expression in liquid-like nuclear condensates[J],2021,118(36).
APA	Pérez-Schindler J..,Kohl B..,Schneider-Heieck K..,Leuchtmann A.B..,Henríquez-Olguín C..,...&Handschin C..(2021).RNA-bound PGC-1α controls gene expression in liquid-like nuclear condensates.Proceedings of the National Academy of Sciences of the United States of America,118(36).
MLA	Pérez-Schindler J.,et al."RNA-bound PGC-1α controls gene expression in liquid-like nuclear condensates".Proceedings of the National Academy of Sciences of the United States of America 118.36(2021).