气候变化领域集成服务门户(CCPortal): The transcription regulator BrsR serves as a network hub of natural competence protein-protein interactions in Streptococcus mutans

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DOI	10.1073/pnas.2106048118
	The transcription regulator BrsR serves as a network hub of natural competence protein-protein interactions in Streptococcus mutans
	Qin H.; Zou Z.; Anderson D.; Sang Y.; Higashi D.; Kreth J.; Merritt J.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:39
英文摘要	Genome evolution is an essential and stringently regulated aspect of biological fitness. For bacteria, natural competence is one of the principal mechanisms of genome evolution and is frequently subject to multiple layers of regulation derived from a plethora of environmental and physiological stimuli. Here, we present a regulatory mechanism that illustrates how such disparate stimuli can be integrated into the Streptococcus mutans natural competence phenotype. S. mutans possesses an intriguing, but poorly understood ability to coordinately control its independently regulated natural competence and bacteriocin genetic pathways as a means to acquire DNA released from closely related, bacteriocinsusceptible streptococci. Our results reveal how the bacteriocinspecific transcription activator BrsR directly mediates this coordination by serving as an anti-adaptor protein responsible for antagonizing the proteolysis of the inherently unstable, natural competence-specific alternative sigma factor ComX. This BrsR ability functions entirely independent of its transcription regulator function and directly modulates the timing and severity of the natural competence phenotype. Additionally, many of the DNA uptake proteins produced by the competence system were surprisingly found to possess adaptor abilities, which are employed to terminate the BrsR regulatory circuit via negative feedback. BrsR- competence protein heteromeric complexes directly inhibit nascent brsR transcription as well as stimulate the Clp-dependent proteolysis of extant BrsR proteins. This study illustrates how critical genetic regulatory abilities can evolve in a potentially limitless variety of proteins without disrupting their conserved ancestral functions. These unrecognized regulatory abilities are likely fundamental for transducing information through complex genetic networks. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Bacteriocin; Gene regulation; Genetic networks; Natural competence; Protein-protein interactions
语种	英语
scopus关键词	bacterial protein; bacteriocin; transcription factor; gene expression regulation; genetics; growth, development and aging; metabolism; protein analysis; Streptococcus mutans; Bacterial Proteins; Bacteriocins; Gene Expression Regulation, Bacterial; Protein Interaction Maps; Streptococcus mutans; Transcription Factors
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251013
作者单位	Department of Restorative Dentistry, Oregon Health and Science University, Portland, OR 97239, United States; Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, United States
推荐引用方式 GB/T 7714	Qin H.,Zou Z.,Anderson D.,et al. The transcription regulator BrsR serves as a network hub of natural competence protein-protein interactions in Streptococcus mutans[J],2021,118(39).
APA	Qin H..,Zou Z..,Anderson D..,Sang Y..,Higashi D..,...&Merritt J..(2021).The transcription regulator BrsR serves as a network hub of natural competence protein-protein interactions in Streptococcus mutans.Proceedings of the National Academy of Sciences of the United States of America,118(39).
MLA	Qin H.,et al."The transcription regulator BrsR serves as a network hub of natural competence protein-protein interactions in Streptococcus mutans".Proceedings of the National Academy of Sciences of the United States of America 118.39(2021).