气候变化领域集成服务门户(CCPortal): IRON MAN interacts with BRUTUS to maintain iron homeostasis in Arabidopsis

CCPortal

DOI	10.1073/pnas.2109063118
	IRON MAN interacts with BRUTUS to maintain iron homeostasis in Arabidopsis
	Li Y.; Lu C.K.; Li C.Y.; Lei R.H.; Pu M.N.; Zhao J.H.; Peng F.; Ping H.Q.; Wang D.; Liang G.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:39
英文摘要	IRON MAN (IMA) peptides, a family of small peptides, control iron (Fe) transport in plants, but their roles in Fe signaling remain unclear. BRUTUS (BTS) is a potential Fe sensor that negatively regulates Fe homeostasis by promoting the ubiquitin-mediated degradation of bHLH105 and bHLH115, two positive regulators of the Fe deficiency response. Here, we show that IMA peptides interact with BTS. The C-terminal parts of IMA peptides contain a conserved BTS interaction domain (BID) that is responsible for their interaction with the C terminus of BTS. Arabidopsis thaliana plants constitutively expressing IMA genes phenocopy the bts-2 mutant. Moreover, IMA peptides are ubiquitinated and degraded by BTS. bHLH105 and bHLH115 also share a BID, which accounts for their interaction with BTS. IMA peptides compete with bHLH105/bHLH115 for interaction with BTS, thereby inhibiting the degradation of these transcription factors by BTS. Genetic analyses suggest that bHLH105/bHLH115 and IMA3 have additive roles and function downstream of BTS. Moreover, the transcription of both BTS and IMA3 is activated directly by bHLH105 and bHLH115 under Fe-deficient conditions. Our findings provide a conceptual framework for understanding the regulation of Fe homeostasis: IMA peptides protect bHLH105/bHLH115 from degradation by sequestering BTS, thereby activating the Fe deficiency response. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Bhlh105; Bhlh115; BTS; IMA; Iron
语种	英语
scopus关键词	carrier protein; iron; IRON MAN peptide; peptides and proteins; protein BRUTUS; unclassified drug; Arabidopsis protein; BRUTUS protein, Arabidopsis; iron; peptide fragment; transcription factor; ubiquitin protein ligase; Arabidopsis thaliana; Article; carboxy terminal sequence; controlled study; gene expression; genetic transcription; IMA gene; iron homeostasis; mutant; nonhuman; protein degradation; protein function; protein protein interaction; ubiquitination; Arabidopsis; gene expression regulation; genetics; growth, development and aging; homeostasis; metabolism; Arabidopsis; Arabidopsis Proteins; Gene Expression Regulation, Plant; Homeostasis; Iron; Peptide Fragments; Transcription Factors; Ubiquitin-Protein Ligases
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251011
作者单位	CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Yunnan, Kunming, 650223, China; Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Mengla, 666303, China; The College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China
推荐引用方式 GB/T 7714	Li Y.,Lu C.K.,Li C.Y.,et al. IRON MAN interacts with BRUTUS to maintain iron homeostasis in Arabidopsis[J],2021,118(39).
APA	Li Y..,Lu C.K..,Li C.Y..,Lei R.H..,Pu M.N..,...&Liang G..(2021).IRON MAN interacts with BRUTUS to maintain iron homeostasis in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America,118(39).
MLA	Li Y.,et al."IRON MAN interacts with BRUTUS to maintain iron homeostasis in Arabidopsis".Proceedings of the National Academy of Sciences of the United States of America 118.39(2021).