气候变化领域集成服务门户(CCPortal): Kinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors

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DOI	10.1073/pnas.2111031118
	Kinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors
	Gysel K.; Laursen M.; Thygesen M.B.; Lironi D.; Bozsóki Z.; Hjuler C.T.; Maolanon N.N.; Cheng J.; Bjørk P.K.; Vinther M.; Madsen L.H.; Rübsam H.; Muszyński A.; Ghodrati A.; Azadi P.; Sullivan J.T.; Ronson C.W.; Jensen K.J.; Blaise M.; Radutoiu S.; Stougaard J.; Andersen K.R.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:44
英文摘要	Plants and animals use cell surface receptors to sense and interpret environmental signals. In legume symbiosis with nitrogen-fixing bacteria, the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases determines compatibility. Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function. We show that the receptor monitors the composition of the amphiphilic LCO molecules and uses kinetic proofreading to control receptor activation and signaling specificity. We demonstrate engineering of the LCO binding site to fine-tune ligand selectivity and correct binding kinetics required for activation of symbiotic signaling in plants. Finally, the hydrophobic patch is found to be a conserved structural signature in this class of LCO receptors across legumes that can be used for in silico predictions. Our results provide insights into the mechanism of cell-surface receptor activation by kinetic proofreading of ligands and highlight the potential in receptor engineering to capture benefits in plant-microbe interactions. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Kinetic proofreading; Legume symbiosis; Lipochitooligosaccharide signaling; LysM receptors; Receptor-ligand interaction
语种	英语
scopus关键词	lipid-linked oligosaccharides; lipopolysaccharide; plant protein; Fabaceae; gene expression; gene expression regulation; genetics; kinetics; metabolism; mycorrhiza; physiology; plant; Rhizobium; signal transduction; symbiosis; Fabaceae; Gene Expression; Gene Expression Regulation, Plant; Kinetics; Lipopolysaccharides; Mycorrhizae; Plant Proteins; Plants; Rhizobium; Signal Transduction; Symbiosis
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250998
作者单位	Department of Molecular Biology and Genetics, Aarhus University, Aarhus C, 8000, Denmark; Department of Chemistry, University of Copenhagen, Frederiksberg, 1871, Denmark; Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, United States; Department of Microbiology and Immunology, University of Otago, Dunedin, 9054, New Zealand; Institut de Recherche en Infectiologie de Montpellier, UMR 9004-CNRS, University of Montpellier, Montpellier, 3429, France
推荐引用方式 GB/T 7714	Gysel K.,Laursen M.,Thygesen M.B.,et al. Kinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors[J],2021,118(44).
APA	Gysel K..,Laursen M..,Thygesen M.B..,Lironi D..,Bozsóki Z..,...&Andersen K.R..(2021).Kinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors.Proceedings of the National Academy of Sciences of the United States of America,118(44).
MLA	Gysel K.,et al."Kinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors".Proceedings of the National Academy of Sciences of the United States of America 118.44(2021).