气候变化领域集成服务门户(CCPortal): Stable lariats bearing a snoRNA (slb-snoRNA) in eukaryotic cells: A level of regulation for guide RNAs

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DOI	10.1073/pnas.2114156118
	Stable lariats bearing a snoRNA (slb-snoRNA) in eukaryotic cells: A level of regulation for guide RNAs
	Talross G.J.S.; Deryusheva S.; Gall J.G.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:45
英文摘要	Small nucleolar (sno)RNAs guide posttranscriptional modifications essential for the biogenesis and function of their target. The majority of snoRNAs in higher eukaryotes are encoded within introns. They are first released from nascent transcripts in the form of a lariat and rapidly targeted by the debranching enzyme and nuclear exonucleases for linearization and further trimming. In this study, we report that some snoRNAs are encoded within unusually stable intronic RNAs. These intronic sequences can escape the debranching enzyme and accumulate as lariats. Stable lariats bearing a snoRNA, or slb-snoRNA, are associated with snoRNA binding proteins but do not guide posttranscriptional modification. While most slb-snoRNAs accumulate in the nucleus, some can be exported to the cytoplasm. We find that this export competes with snoRNA maturation. Slb-snoRNAs provide a previously unknown layer of regulation to snoRNA and snoRNA binding proteins. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Guide activity; RNA modification; SnoRNA; Stable lariat
语种	英语
scopus关键词	small nucleolar RNA; guide RNA; small nucleolar RNA; animal cell; Article; bioaccumulation; bioinformatics; cell culture; coimmunoprecipitation; controlled study; cytoplasm; DNA modification; eukaryotic cell; female; gene control; genetic association; genetic code; genetic transfection; human; human cell; microinjection; mouse; nonhuman; Northern blotting; oocyte; protein RNA binding; real time polymerase chain reaction; RNA processing; RNA purification; RNA sequencing; RNA stability; 3T3 cell line; animal; gene expression regulation; HeLa cell line; intron; metabolism; Saccharomyces cerevisiae; Xenopus laevis; 3T3 Cells; Animals; Female; Gene Expression Regulation; HeLa Cells; Humans; Introns; Mice; RNA, Circular; RNA, Guide; RNA, Small Nucleolar; Saccharomyces cerevisiae; Xenopus laevis
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250994
作者单位	Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, United States
推荐引用方式 GB/T 7714	Talross G.J.S.,Deryusheva S.,Gall J.G.. Stable lariats bearing a snoRNA (slb-snoRNA) in eukaryotic cells: A level of regulation for guide RNAs[J],2021,118(45).
APA	Talross G.J.S.,Deryusheva S.,&Gall J.G..(2021).Stable lariats bearing a snoRNA (slb-snoRNA) in eukaryotic cells: A level of regulation for guide RNAs.Proceedings of the National Academy of Sciences of the United States of America,118(45).
MLA	Talross G.J.S.,et al."Stable lariats bearing a snoRNA (slb-snoRNA) in eukaryotic cells: A level of regulation for guide RNAs".Proceedings of the National Academy of Sciences of the United States of America 118.45(2021).