气候变化领域集成服务门户(CCPortal): The Shh/Gli3 gene regulatory network precedes the origin of paired fins and reveals the deep homology between distal fins and digits

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DOI	10.1073/pnas.2100575118
	The Shh/Gli3 gene regulatory network precedes the origin of paired fins and reveals the deep homology between distal fins and digits
	Letelier J.; Naranjo S.; Sospedra-Arrufat I.; Ramon Martinez-Morales J.; Lopez-Rios J.; Shubin N.; Gomez-Skarmeta J.L.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:46
英文摘要	One of the central problems of vertebrate evolution is understanding the relationship among the distal portions of fins and limbs. Lacking comparable morphological markers of these regions in fish and tetrapods, these relationships have remained uncertain for the past century and a half. Here we show that Gli3 functions in controlling the proliferative expansion of distal progenitors are shared among dorsal and paired fins as well as tetrapod limbs. Mutant knockout gli3 fins in medaka (Oryzias latipes) form multiple radials and rays, in a pattern reminiscent of the polydactyly observed in Gli3-null mutant mice. In limbs, Gli3 controls both anterior-posterior patterning and cell proliferation, two processes that can be genetically uncoupled. In situ hybridization, quantification of proliferation markers, and analysis of regulatory regions reveal that in paired and dorsal fins, gli3 plays a main role in controlling proliferation but not in patterning. Moreover, gli3 downregulation in shh mutant fins rescues fin loss in a manner similar to how Gli3 deficiency restores digits in the limbs of Shh mutant mouse embryos. We hypothesize that the Gli3/Shh gene pathway preceded the origin of paired appendages and was originally involved in modulating cell proliferation. Accordingly, the distal regions of dorsal fins, paired fins, and limbs retain a deep regulatory and functional homology that predates the origin of paired appendages. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Fin growth; Fin-to-limb transition; Gli3; Shh
语种	英语
scopus关键词	animal tissue; Article; body patterning; cell proliferation; controlled study; digit (body part); down regulation; embryo; fin (organ); fish; gene; gene function; gene regulatory network; Gli3 gene; mouse; mutant; nonhuman; Oryzias latipes; Shh gene; animal; body patterning; evolution; fin (organ); gene expression regulation; gene regulatory network; genetics; growth, development and aging; limb; Oryzias; fish protein; Gli3 protein, mouse; nerve protein; transcriptional activator GLI3; Animal Fins; Animals; Biological Evolution; Body Patterning; Cell Proliferation; Extremities; Fish Proteins; Gene Expression Regulation, Developmental; Gene Regulatory Networks; Mice; Nerve Tissue Proteins; Oryzias; Zinc Finger Protein Gli3
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250992
作者单位	Centro Andaluz de Biologia del Desarrollo, Consejo Superior de Investigaciones Cientificas, Universidad Pablo de Olavide, and Junta de Andalucia, Sevilla, 41013, Spain; Center for Integrative Biology, Facultad de Ciencias, Universidad Mayor, Santiago, 8580745, Chile; Department of Organismal Biology and Anatomy, University of Chicago, Chicago, IL 60637, United States
推荐引用方式 GB/T 7714	Letelier J.,Naranjo S.,Sospedra-Arrufat I.,et al. The Shh/Gli3 gene regulatory network precedes the origin of paired fins and reveals the deep homology between distal fins and digits[J],2021,118(46).
APA	Letelier J..,Naranjo S..,Sospedra-Arrufat I..,Ramon Martinez-Morales J..,Lopez-Rios J..,...&Gomez-Skarmeta J.L..(2021).The Shh/Gli3 gene regulatory network precedes the origin of paired fins and reveals the deep homology between distal fins and digits.Proceedings of the National Academy of Sciences of the United States of America,118(46).
MLA	Letelier J.,et al."The Shh/Gli3 gene regulatory network precedes the origin of paired fins and reveals the deep homology between distal fins and digits".Proceedings of the National Academy of Sciences of the United States of America 118.46(2021).