气候变化领域集成服务门户(CCPortal): Dynamic competition between a ligand and transcription factor NusA governs riboswitch-mediated transcription regulation

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DOI	10.1073/pnas.2109026118
	Dynamic competition between a ligand and transcription factor NusA governs riboswitch-mediated transcription regulation
	Chauvier A.; Ajmera P.; Yadav R.; Walter N.G.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:47
英文摘要	Cotranscriptional RNA folding is widely assumed to influence the timely control of gene expression, but our understanding remains limited. In bacteria, the fluoride (F2)-sensing riboswitch is a transcriptional control element essential to defend against toxic F2 levels. Using this model riboswitch, we find that its ligand F2 and essential bacterial transcription factor NusA compete to bind the cotranscriptionally folding RNA, opposing each other's modulation of downstream pausing and termination by RNA polymerase. Single-molecule fluorescence assays probing active transcription elongation complexes discover that NusA unexpectedly binds highly reversibly, frequently interrogating the complex for emerging, cotranscriptionally folding RNA duplexes. NusA thus fine-tunes the transcription rate in dependence of the ligandresponsive higher-order structure of the riboswitch. At the high NusA concentrations found intracellularly, this dynamic modulation is expected to lead to adaptive bacterial transcription regulation with fast response times. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cotranscriptional folding; NusA; Riboswitch; RNA polymerase pausing; Single-molecule fluorescence microscopy
语种	英语
scopus关键词	ligand; RNA polymerase; transcription factor; transcription factor NusA; unclassified drug; bacterial RNA; DNA directed RNA polymerase; Escherichia coli protein; nusA protein, E coli; transcription elongation factor; transcription factor; Article; binding site; complex formation; fluorescence analysis; ligand binding; protein expression; riboswitch; sequence analysis; thermodynamics; transcription elongation; transcription regulation; chemistry; Escherichia coli; gene expression regulation; genetic transcription; genetics; metabolism; RNA folding; DNA-Directed RNA Polymerases; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Ligands; Riboswitch; RNA Folding; RNA, Bacterial; Transcription Factors; Transcription, Genetic; Transcriptional Elongation Factors
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250972
作者单位	Single Molecule Analysis Group, University of Michigan, Ann Arbor, MI 48109, United States; Center for RNA Biomedicine, Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, United States
推荐引用方式 GB/T 7714	Chauvier A.,Ajmera P.,Yadav R.,et al. Dynamic competition between a ligand and transcription factor NusA governs riboswitch-mediated transcription regulation[J],2021,118(47).
APA	Chauvier A.,Ajmera P.,Yadav R.,&Walter N.G..(2021).Dynamic competition between a ligand and transcription factor NusA governs riboswitch-mediated transcription regulation.Proceedings of the National Academy of Sciences of the United States of America,118(47).
MLA	Chauvier A.,et al."Dynamic competition between a ligand and transcription factor NusA governs riboswitch-mediated transcription regulation".Proceedings of the National Academy of Sciences of the United States of America 118.47(2021).