气候变化领域集成服务门户(CCPortal): Antagonistic cotranscriptional regulation through ARGONAUTE1 and the THO/TREX complex orchestrates FLC transcriptional output

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DOI	10.1073/pnas.2113757118
	Antagonistic cotranscriptional regulation through ARGONAUTE1 and the THO/TREX complex orchestrates FLC transcriptional output
	Xu C.; Fang X.; Lu T.; Dean C.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:47
英文摘要	Quantitative transcriptional control is essential for physiological and developmental processes in many organisms. Transcriptional output is influenced by cotranscriptional processes interconnected to chromatin regulation, but how the functions of different cotranscriptional regulators are integrated is poorly understood. The Arabidopsis floral repressor locus FLOWERING LOCUS C (FLC) is cotranscriptionally repressed by alternative processing of the antisense transcript COOLAIR. Proximal 30-end processing of COOLAIR resolves a cotranscriptionally formed R-loop, and this process physically links to a histone-modifying complex FLD/SDG26/LD. This induces a chromatin environment locally that determines low transcription initiation and a slow elongation rate to both sense and antisense strands. Here, we show that ARGONAUTE1 (AGO1) genetically functions in this cotranscriptional repression mechanism. AGO1 associates with COOLAIR and influences COOLAIR splicing dynamics to promote proximal COOLAIR, R-loop resolution, and chromatin silencing. Proteomic analyses revealed physical associations between AGO1, subunits of RNA Polymerase II (Pol II), the splicing-related proteins-the spliceosome NineTeen Complex (NTC) and related proteins (NTR)-and the THO/TREX complex. We connect these activities by demonstrating that the THO/TREX complex activates FLC expression acting antagonistically to AGO1 in COOLAIR processing. Together these data reveal that antagonistic cotranscriptional regulation through AGO1 or THO/TREX influences COOLAIR processing to deliver a local chromatin environment that determines FLC transcriptional output. The involvement of these conserved cotranscriptional regulators suggests similar mechanisms may underpin quantitative transcriptional regulation generally. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	ARGONAUTE1; Cotranscription; RNA processing; THO/TREX complex
语种	英语
scopus关键词	argonaute 1 protein; protein THO; protein TREX; RNA polymerase II; unclassified drug; AGO1 protein, Arabidopsis; Arabidopsis protein; argonaute protein; complementary RNA; flowering locus D protein, Arabidopsis; histone; histone deacetylase; histone lysine methyltransferase; LD protein, Arabidopsis; MADS domain protein; RNA polymerase II; SDG26 protein, Arabidopsis; Article; chromatin; controlled study; COOLAIR gene; enzyme activity; flowering; FLOWERING LOCUS C gene; gene expression; gene function; gene repression; gene silencing; nonhuman; plant gene; protein analysis; protein processing; RNA splicing; transcription regulation; Arabidopsis; cell nucleus; flower; gene expression regulation; genetics; metabolism; proteomics; Arabidopsis; Arabidopsis Proteins; Argonaute Proteins; Cell Nucleus; Chromatin; Flowers; Gene Expression Regulation, Plant; Gene Silencing; Histone Deacetylases; Histone-Lysine N-Methyltransferase; Histones; MADS Domain Proteins; Proteomics; RNA Polymerase II; RNA Splicing; RNA, Antisense
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250971
作者单位	Department of Cell and Developmental Biology, John Innes Centre, Norwich, NR4 7UH, United Kingdom
推荐引用方式 GB/T 7714	Xu C.,Fang X.,Lu T.,et al. Antagonistic cotranscriptional regulation through ARGONAUTE1 and the THO/TREX complex orchestrates FLC transcriptional output[J],2021,118(47).
APA	Xu C.,Fang X.,Lu T.,&Dean C..(2021).Antagonistic cotranscriptional regulation through ARGONAUTE1 and the THO/TREX complex orchestrates FLC transcriptional output.Proceedings of the National Academy of Sciences of the United States of America,118(47).
MLA	Xu C.,et al."Antagonistic cotranscriptional regulation through ARGONAUTE1 and the THO/TREX complex orchestrates FLC transcriptional output".Proceedings of the National Academy of Sciences of the United States of America 118.47(2021).