气候变化领域集成服务门户(CCPortal): Heme auxotrophy in abundant aquatic microbial lineages

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DOI	10.1073/pnas.2102750118
	Heme auxotrophy in abundant aquatic microbial lineages
	Kim S.; Kang I.; Lee J.-W.; Jeon C.O.; Giovannoni S.J.; Cho J.-C.
发表日期	2021
ISSN	1091-6490
卷号	118 期号:47
英文摘要	Heme, a porphyrin ring complexed with iron, is a metalloprosthetic group of numerous proteins involved in diverse metabolic and respiratory processes across all domains of life, and is thus considered essential for respiring organisms. Several microbial groups are known to lack the de novo heme biosynthetic pathway and therefore require exogenous heme from the environment. These heme auxotroph groups are largely limited to pathogens, symbionts, or microorganisms living in nutrient-replete conditions, whereas the complete absence of heme biosynthesis is extremely rare in free-living organisms. Here, we show that the acI lineage, a predominant and ubiquitous free-living bacterial group in freshwater habitats, is auxotrophic for heme, based on the experimental or genomic evidence. We found that two recently cultivated acI isolates require exogenous heme for their growth. One of the cultured acI isolates also exhibited auxotrophy for riboflavin. According to whole-genome analyses, all (n = 20) isolated acI strains lacked essential enzymes necessary for heme biosynthesis, indicating that heme auxotrophy is a conserved trait in this lineage. Analyses of >24,000 representative genomes for species clusters of the Genome Taxonomy Database revealed that heme auxotrophy is widespread across abundant but not-yet-cultivated microbial groups, including Patescibacteria, Marinisomatota (SAR406), Actinomarinales (OM1), and Marine groups IIb and III of Euryarchaeota Our findings indicate that heme auxotrophy is a more common phenomenon than previously thought, and may lead to use of heme as a growth factor to increase the cultured microbial diversity.
英文关键词	acI lineage; auxotrophy; Black Queen Hypothesis; heme; riboflavin
语种	英语
scopus关键词	fresh water; heme; riboflavin; archaeon; bacterial genome; bacterium; biodiversity; biosynthesis; ecosystem; genetics; metabolism; microbiology; Archaea; Bacteria; Biodiversity; Biosynthetic Pathways; Ecosystem; Fresh Water; Genome, Bacterial; Heme; Riboflavin
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250970
作者单位	Department of Biological Sciences, Inha UniversityIncheon 22212, South Korea; Center for Molecular and Cell Biology, Inha University, Incheon 22212, Republic of Korea; chojc@inha.ac.kr; Department of Life Science, Hanyang UniversitySeoul 04763, South Korea; Department of Life Science, Chung-Ang UniversitySeoul 06974, South Korea; Department of Microbiology, Oregon State University, OR 97331, Corvallis, United States; Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea; ikang@inha.ac.kr chojc@inha.ac.kr; Department of Biological Sciences and Bioengineering, Inha UniversityIncheon 22212, South Korea
推荐引用方式 GB/T 7714	Kim S.,Kang I.,Lee J.-W.,et al. Heme auxotrophy in abundant aquatic microbial lineages[J],2021,118(47).
APA	Kim S.,Kang I.,Lee J.-W.,Jeon C.O.,Giovannoni S.J.,&Cho J.-C..(2021).Heme auxotrophy in abundant aquatic microbial lineages.Proceedings of the National Academy of Sciences of the United States of America,118(47).
MLA	Kim S.,et al."Heme auxotrophy in abundant aquatic microbial lineages".Proceedings of the National Academy of Sciences of the United States of America 118.47(2021).