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DOI | 10.1073/pnas.2102750118 |
Heme auxotrophy in abundant aquatic microbial lineages | |
Kim S.; Kang I.; Lee J.-W.; Jeon C.O.; Giovannoni S.J.; Cho J.-C. | |
发表日期 | 2021 |
ISSN | 1091-6490 |
卷号 | 118期号:47 |
英文摘要 | Heme, a porphyrin ring complexed with iron, is a metalloprosthetic group of numerous proteins involved in diverse metabolic and respiratory processes across all domains of life, and is thus considered essential for respiring organisms. Several microbial groups are known to lack the de novo heme biosynthetic pathway and therefore require exogenous heme from the environment. These heme auxotroph groups are largely limited to pathogens, symbionts, or microorganisms living in nutrient-replete conditions, whereas the complete absence of heme biosynthesis is extremely rare in free-living organisms. Here, we show that the acI lineage, a predominant and ubiquitous free-living bacterial group in freshwater habitats, is auxotrophic for heme, based on the experimental or genomic evidence. We found that two recently cultivated acI isolates require exogenous heme for their growth. One of the cultured acI isolates also exhibited auxotrophy for riboflavin. According to whole-genome analyses, all (n = 20) isolated acI strains lacked essential enzymes necessary for heme biosynthesis, indicating that heme auxotrophy is a conserved trait in this lineage. Analyses of >24,000 representative genomes for species clusters of the Genome Taxonomy Database revealed that heme auxotrophy is widespread across abundant but not-yet-cultivated microbial groups, including Patescibacteria, Marinisomatota (SAR406), Actinomarinales (OM1), and Marine groups IIb and III of Euryarchaeota Our findings indicate that heme auxotrophy is a more common phenomenon than previously thought, and may lead to use of heme as a growth factor to increase the cultured microbial diversity. |
英文关键词 | acI lineage; auxotrophy; Black Queen Hypothesis; heme; riboflavin |
语种 | 英语 |
scopus关键词 | fresh water; heme; riboflavin; archaeon; bacterial genome; bacterium; biodiversity; biosynthesis; ecosystem; genetics; metabolism; microbiology; Archaea; Bacteria; Biodiversity; Biosynthetic Pathways; Ecosystem; Fresh Water; Genome, Bacterial; Heme; Riboflavin |
来源期刊 | Proceedings of the National Academy of Sciences of the United States of America |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/250970 |
作者单位 | Department of Biological Sciences, Inha UniversityIncheon 22212, South Korea; Center for Molecular and Cell Biology, Inha University, Incheon 22212, Republic of Korea; chojc@inha.ac.kr; Department of Life Science, Hanyang UniversitySeoul 04763, South Korea; Department of Life Science, Chung-Ang UniversitySeoul 06974, South Korea; Department of Microbiology, Oregon State University, OR 97331, Corvallis, United States; Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea; ikang@inha.ac.kr chojc@inha.ac.kr; Department of Biological Sciences and Bioengineering, Inha UniversityIncheon 22212, South Korea |
推荐引用方式 GB/T 7714 | Kim S.,Kang I.,Lee J.-W.,et al. Heme auxotrophy in abundant aquatic microbial lineages[J],2021,118(47). |
APA | Kim S.,Kang I.,Lee J.-W.,Jeon C.O.,Giovannoni S.J.,&Cho J.-C..(2021).Heme auxotrophy in abundant aquatic microbial lineages.Proceedings of the National Academy of Sciences of the United States of America,118(47). |
MLA | Kim S.,et al."Heme auxotrophy in abundant aquatic microbial lineages".Proceedings of the National Academy of Sciences of the United States of America 118.47(2021). |
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