气候变化领域集成服务门户(CCPortal): Host genotype structures the microbiome of a globally dispersed marine phytoplankton

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DOI	10.1073/pnas.2105207118
	Host genotype structures the microbiome of a globally dispersed marine phytoplankton
	Ahern O.M.; Whittaker K.A.; Williams T.C.; Hunt D.E.; Rynearson T.A.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:48
英文摘要	Phytoplankton support complex bacterial microbiomes that rely on phytoplankton-derived extracellular compounds and perform functions necessary for algal growth. Recent work has revealed sophisticated interactions and exchanges of molecules between specific phytoplankton-bacteria pairs, but the role of host genotype in regulating those interactions is unknown. Here, we show how phytoplankton microbiomes are shaped by intraspecific genetic variation in the host using global environmental isolates of the model phytoplankton host Thalassiosira rotula and a laboratory common garden experiment. A set of 81 environmental T. rotula genotypes from three ocean basins and eight genetically distinct populations did not reveal a core microbiome. While no single bacterial phylotype was shared across all genotypes, we found strong genotypic influence of T. rotula, with microbiomes associating more strongly with host genetic population than with environmental factors. The microbiome association with host genetic population persisted across different ocean basins, suggesting that microbiomes may be associated with host populations for decades. To isolate the impact of host genotype on microbiomes, a common garden experiment using eight genotypes from three distinct host populations again found that host genotype influenced microbial community composition, suggesting that a process we describe as genotypic filtering, analogous to environmental filtering, shapes phytoplankton microbiomes. In both the environmental and laboratory studies, microbiome variation between genotypes suggests that other factors influenced microbiome composition but did not swamp the dominant signal of host genetic background. The long-term association of microbiomes with specific host genotypes reveals a possible mechanism explaining the evolution and maintenance of complex phytoplankton-bacteria chemical exchanges. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Host-microbe interactions; Microbiome; Phycosphere; Phytoplankton-bacteria interactions; Population genetics
语种	英语
scopus关键词	article; environmental factor; filtration; genetic background; genetic variation; host microbe interaction; microbial community; nonhuman; phytoplankton; population genetics; sea; swamp; Thalassiosira; bacterium; diatom; ecosystem; genetics; genotype; microbiology; microflora; phytoplankton; population genetics; procedures; RNA 16S; Bacteria; Diatoms; Ecosystem; Genetics, Population; Genotype; Microbiota; Phytoplankton; RNA, Ribosomal, 16S
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250966
作者单位	Graduate School of Oceanography, University of Rhode Island, Narragansett, RI 02882, United States; Nicholas School of the Environment, Duke University Marine Laboratory, Beaufort, NC 28516, United States
推荐引用方式 GB/T 7714	Ahern O.M.,Whittaker K.A.,Williams T.C.,et al. Host genotype structures the microbiome of a globally dispersed marine phytoplankton[J],2021,118(48).
APA	Ahern O.M.,Whittaker K.A.,Williams T.C.,Hunt D.E.,&Rynearson T.A..(2021).Host genotype structures the microbiome of a globally dispersed marine phytoplankton.Proceedings of the National Academy of Sciences of the United States of America,118(48).
MLA	Ahern O.M.,et al."Host genotype structures the microbiome of a globally dispersed marine phytoplankton".Proceedings of the National Academy of Sciences of the United States of America 118.48(2021).