气候变化领域集成服务门户(CCPortal): Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis

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DOI	10.1073/pnas.2105021118
	Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis
	Abdelrahman M.; Nishiyama R.; Tran C.D.; Kusano M.; Nakabayashi R.; Okazaki Y.; Matsuda F.; Chávez Montes R.A.; Mostofa M.G.; Li W.; Watanabe Y.; Fukushima A.; Tanaka M.; Seki M.; Saito K.; Herrera-Estrella L.; Tran L.-S.P.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:48
英文摘要	Cytokinin (CK) in plants regulates both developmental processes and adaptation to environmental stresses. Arabidopsis histidine phosphotransfer ahp2,3,5 and type-B Arabidopsis response regulator arr1,10,12 triple mutants are almost completely defective in CK signaling, and the ahp2,3,5 mutant was reported to be salt tolerant. Here, we demonstrate that the arr1,10,12 mutant is also more tolerant to salt stress than wild-type (WT) plants. A comprehensive metabolite profiling coupled with transcriptome analysis of the ahp2,3,5 and arr1,10,12 mutants was conducted to elucidate the salt tolerance mechanisms mediated by CK signaling. Numerous primary (e.g., sugars, amino acids, and lipids) and secondary (e.g., flavonoids and sterols) metabolites accumulated in these mutants under nonsaline and saline conditions, suggesting that both prestress and poststress accumulations of stress-related metabolites contribute to improved salt tolerance in CK-signaling mutants. Specifically, the levels of sugars (e.g., trehalose and galactinol), amino acids (e.g., branched-chain amino acids and γ-aminobutyric acid), anthocyanins, sterols, and unsaturated triacylglycerols were higher in the mutant plants than in WT plants. Notably, the reprograming of flavonoid and lipid pools was highly coordinated and concomitant with the changes in transcriptional levels, indicating that these metabolic pathways are transcriptionally regulated by CK signaling. The discovery of the regulatory role of CK signaling on membrane lipid reprogramming provides a greater understanding of CK-mediated salt tolerance in plants. This knowledge will contribute to the development of salt-tolerant crops with the ability to withstand salinity as a key driver to ensure global food security in the era of climate crisis. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Comparative metabolomics; Comparative transcriptomics; Cytokinin signaling; Regulatory network; Salt stress
语种	英语
scopus关键词	4 aminobutyric acid; anthocyanin; branched chain amino acid; cytokinin; flavonoid; lipid; sterol; sugar; trehalose; Arabidopsis protein; cytokinin; Arabidopsis; Article; gene regulatory network; metabolite; metabolomics; mutant; nonhuman; salt stress; salt tolerance; signal transduction; wild type; adaptation; Arabidopsis; drug effect; gene expression; gene expression profiling; gene expression regulation; genetics; lipid metabolism; metabolism; physiological stress; physiology; procedures; salinity; salt stress; salt tolerance; signal transduction; Adaptation, Physiological; Arabidopsis; Arabidopsis Proteins; Cytokinins; Flavonoids; Gene Expression; Gene Expression Profiling; Gene Expression Regulation; Gene Expression Regulation, Plant; Gene Regulatory Networks; Lipid Metabolism; Lipids; Metabolomics; Salinity; Salt Stress; Salt Tolerance; Signal Transduction; Stress, Physiological
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250963
作者单位	Faculty of Science, Galala University, Suze, El Sokhna43511, Egypt; Botany Department, Faculty of Science, Aswan University, Aswan, 81528, Egypt; Stress Adaptation Research Unit, RIKEN Center for Sustainable Resource Science, Yokohama, 230-0045, Japan; Metabolomics Research Group, RIKEN Center for Sustainable Resource Science, Yokohama, 230-0045, Japan; Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8572, Japan; Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Osaka, 565-0871, Japan; Department of Plant and Soil Science, Institute of Genomics for Crop Abiotic Stress Tolerance, Texas Tech University, Lubbock, TX 79409, United States; Department of Biochemistry and Molecular Biology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh; State Key Laboratory of Cotton Biology, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Hen...
推荐引用方式 GB/T 7714	Abdelrahman M.,Nishiyama R.,Tran C.D.,et al. Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis[J],2021,118(48).
APA	Abdelrahman M..,Nishiyama R..,Tran C.D..,Kusano M..,Nakabayashi R..,...&Tran L.-S.P..(2021).Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America,118(48).
MLA	Abdelrahman M.,et al."Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity in Arabidopsis".Proceedings of the National Academy of Sciences of the United States of America 118.48(2021).