气候变化领域集成服务门户(CCPortal): An endoplasmic reticulum–localized cytochrome b5 regulates high-affinity K+ transport in response to salt stress in rice

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DOI	10.1073/pnas.2114347118
	An endoplasmic reticulum–localized cytochrome b5 regulates high-affinity K+ transport in response to salt stress in rice
	Song T.; Shi Y.; Shen L.; Cao C.; Shen Y.; Jing W.; Tian Q.; Lin F.; Li W.; Zhang W.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:50
英文摘要	Potassium (K+) is an essential element for growth and development in both animals and plants, while high levels of environmental sodium (Na+) represent a threat to most plants. The uptake of K+ from high-saline environments is an essential mechanism to maintain intracellular K+/Na+ homeostasis, which can help reduce toxicity caused by Na+ accumulation, thereby improving the salt tolerance of plants. However, the mechanisms and regulation of K+-uptake during salt stress remain poorly understood. In this study, we identified an endoplasmic reticulum–localized cytochrome b5 (OsCYB5-2) that interacted with a high-affinity K+ transporter (OsHAK21) at the plasma membrane. The association of OsCYB5-2 with the OsHAK21 transporter caused an increase in transporter activity by enhancing the apparent affinity for K+binding but not Na+-binding. Heme binding to OsCYB5-2 was essential for the regulation of OsHAK21. High salinity directly triggered the OsHAK21–OsCYB5-2 interaction, promoting OsHAK21-mediated K+-uptake and restricting Na+ entry into cells; this maintained intracellular K+/Na+ homeostasis in rice cells. Finally, overexpression of OsCYB5-2 increased OsHAK21-mediated K+ transport and improved salt tolerance in rice seedlings. This study revealed a posttranslational regulatory mechanism for HAK transporter activity mediated by a cytochrome b5 and highlighted the coordinated action of two proteins to perceive Na+ in response to salt stress. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cytochrome b5; HAK transporter; Ion homeostasis; Rice; Salt tolerance
语种	英语
scopus关键词	adenosine triphosphatase (potassium sodium); cytochrome b5; heme binding protein; potassium channel; protein OsCYB5 2; protein OsHAK21; sodium channel; unclassified drug; cytochrome b; plant protein; sodium; Article; cell membrane; controlled study; endoplasmic reticulum; enzyme activity; enzyme regulation; nonhuman; plant cell; potassium transport; protein processing; protein protein interaction; rice; salt stress; salt tolerance; seedling; drug effect; gene expression regulation; genetics; metabolism; Oryza; plant root; salinity; salt stress; shoot; Cytochromes b; Gene Expression Regulation, Plant; Oryza; Plant Proteins; Plant Roots; Plant Shoots; Salinity; Salt Stress; Seedlings; Sodium
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250931
作者单位	College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China
推荐引用方式 GB/T 7714	Song T.,Shi Y.,Shen L.,等. An endoplasmic reticulum–localized cytochrome b5 regulates high-affinity K+ transport in response to salt stress in rice[J],2021,118(50).
APA	Song T..,Shi Y..,Shen L..,Cao C..,Shen Y..,...&Zhang W..(2021).An endoplasmic reticulum–localized cytochrome b5 regulates high-affinity K+ transport in response to salt stress in rice.Proceedings of the National Academy of Sciences of the United States of America,118(50).
MLA	Song T.,et al."An endoplasmic reticulum–localized cytochrome b5 regulates high-affinity K+ transport in response to salt stress in rice".Proceedings of the National Academy of Sciences of the United States of America 118.50(2021).