| DOI | 10.1073/pnas.2112836118
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| Adaptations in metabolism and protein translation give rise to the Crabtree effect in yeast |
| Malina C.; Yu R.; Bjorkeroth J.; Kerkhoven E.J.; Nielsen J.
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| 发表日期 | 2021
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| ISSN | 0027-8424
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| 卷号 | 118期号:51 |
| 英文摘要 | Aerobic fermentation, also referred to as the Crabtree effect in yeast, is a well-studied phenomenon that allows many eukaryal cells to attain higher growth rates at high glucose availability. Not all yeasts exhibit the Crabtree effect, and it is not known why Crabtree-negative yeasts can grow at rates comparable to Crabtree-positive yeasts. Here, we quantitatively compared two Crabtree-positive yeasts, Saccharomyces cerevisiae and Schizosaccharomyces pombe, and two Crabtree-negative yeasts, Kluyveromyces marxianus and Scheffersomyces stipitis, cultivated under glucose excess conditions. Combining physiological and proteome quantification with genome-scale metabolic modeling, we found that the two groups differ in energy metabolism and translation efficiency. In Crabtree-positive yeasts, the central carbon metabolism flux and proteome allocation favor a glucose utilization strategy minimizing proteome cost as proteins translation parameters, including ribosomal content and/or efficiency, are lower. Crabtree-negative yeasts, however, use a strategy of maximizing ATP yield, accompanied by higher protein translation parameters. Our analyses provide insight into the underlying reasons for the Crabtree effect, demonstrating a coupling to adaptations in both metabolism and protein translation. © 2021 National Academy of Sciences. All rights reserved. |
| 英文关键词 | Constraint-based modeling; Crabtree effect; Proteomics; Systems biology; Yeast |
| 语种 | 英语
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| scopus关键词 | proteome; fungal protein; glucose; proteome; proton transporting adenosine triphosphate synthase; Article; carbon metabolism; controlled study; Crabtree effect; energy metabolism; Kluyveromyces marxianus; metabolism; nonhuman; protein processing; proteomics; quantitative analysis; Saccharomyces cerevisiae; Scheffersomyces stipitis; Schizosaccharomyces pombe; systems biology; yeast; aerobic metabolism; fermentation; gene expression regulation; genetics; physiology; species difference; Aerobiosis; Fermentation; Fungal Proteins; Gene Expression Regulation, Fungal; Glucose; Mitochondrial Proton-Translocating ATPases; Proteome; Species Specificity; Yeasts |
| 来源期刊 | Proceedings of the National Academy of Sciences of the United States of America
(IF:9.58[JCR-2018],10.6[5-Year]) |
| 文献类型 | 期刊论文
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| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/250906
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| 作者单位 | Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, SE-412 96, Sweden; Wallenberg Center for Protein Research, Chalmers University of Technology, Gothenburg, SE-412 96, Sweden; Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, Gothenburg, SE-412 96, Sweden; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, DK-2800, Denmark; BioInnovation Institute, Copenhagen, DK-2200, Denmark
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推荐引用方式
GB/T 7714 |
Malina C.,Yu R.,Bjorkeroth J.,et al. Adaptations in metabolism and protein translation give rise to the Crabtree effect in yeast[J],2021,118(51).
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| APA |
Malina C.,Yu R.,Bjorkeroth J.,Kerkhoven E.J.,&Nielsen J..(2021).Adaptations in metabolism and protein translation give rise to the Crabtree effect in yeast.Proceedings of the National Academy of Sciences of the United States of America,118(51).
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| MLA |
Malina C.,et al."Adaptations in metabolism and protein translation give rise to the Crabtree effect in yeast".Proceedings of the National Academy of Sciences of the United States of America 118.51(2021).
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