气候变化领域集成服务门户(CCPortal): Spatiotemporal dynamics of SETD5-containing NCoR–HDAC3 complex determines enhancer activation for adipogenesis

CCPortal

DOI	10.1038/s41467-021-27321-5
	Spatiotemporal dynamics of SETD5-containing NCoR–HDAC3 complex determines enhancer activation for adipogenesis
	Matsumura Y.; Ito R.; Yajima A.; Yamaguchi R.; Tanaka T.; Kawamura T.; Magoori K.; Abe Y.; Uchida A.; Yoneshiro T.; Hirakawa H.; Zhang J.; Arai M.; Yang C.; Yang G.; Takahashi H.; Fujihashi H.; Nakaki R.; Yamamoto S.; Ota S.; Tsutsumi S.; Inoue S.-I.; Kimura H.; Wada Y.; Kodama T.; Inagaki T.; Osborne T.F.; Aburatani H.; Node K.; Sakai J.
发表日期	2021
ISSN	2041-1723
卷号	12 期号:1
英文摘要	Enhancer activation is essential for cell-type specific gene expression during cellular differentiation, however, how enhancers transition from a hypoacetylated “primed” state to a hyperacetylated-active state is incompletely understood. Here, we show SET domain-containing 5 (SETD5) forms a complex with NCoR-HDAC3 co-repressor that prevents histone acetylation of enhancers for two master adipogenic regulatory genes Cebpa and Pparg early during adipogenesis. The loss of SETD5 from the complex is followed by enhancer hyperacetylation. SETD5 protein levels were transiently increased and rapidly degraded prior to enhancer activation providing a mechanism for the loss of SETD5 during the transition. We show that induction of the CDC20 co-activator of the ubiquitin ligase leads to APC/C mediated degradation of SETD5 during the transition and this operates as a molecular switch that facilitates adipogenesis. © 2021, The Author(s).
语种	英语
scopus关键词	cell component; gene; gene expression; histology; protein; anaphase promoting complex; CCAAT enhancer binding protein; Cdc20 protein, mouse; CEBPA protein, mouse; cell cycle protein 20; histone; histone deacetylase; histone deacetylase 3; methyltransferase; Ncor1 protein, mouse; peroxisome proliferator activated receptor gamma; Pparg protein, mouse; protein binding; receptor interacting protein 140; SETD5 protein, mouse; 3T3-L1 cell line; acetylation; adipogenesis; animal; enhancer region; gene expression regulation; genetics; HEK293 cell line; human; metabolism; mouse; nude mouse; protein degradation; Sf9 cell line; signal transduction; 3T3-L1 Cells; Acetylation; Adipogenesis; Anaphase-Promoting Complex-Cyclosome; Animals; CCAAT-Enhancer-Binding Proteins; Cdc20 Proteins; Enhancer Elements, Genetic; Gene Expression Regulation; HEK293 Cells; Histone Deacetylases; Histones; Humans; Methyltransferases; Mice; Mice, Nude; Nuclear Receptor Co-Repressor 1; PPAR gamma; Protein Binding; Proteolysis; Sf9 Cells; Signal Transduction
来源期刊	Nature Communications
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250558
作者单位	Division of Metabolic Medicine, Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan; Division of Molecular Physiology and Metabolism, Tohoku University Graduate School of Medicine, Sendai, Japan; Department of Cardiovascular Medicine, Saga University, Saga, Japan; Department of Nuclear Receptor Medicine, Laboratories for Systems Biology and Medicine, Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan; Isotope Science Center, The University of Tokyo, Tokyo, Japan; Department of Physiology and Cell Biology, Tokyo Medical and Dental University (TMDU), Graduate School, Tokyo, Japan; Genome Science Division, Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan; Rhelixa Inc, Tokyo, Japan; Cell Biology Center, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama, Japan; Laboratory of Epigenetics and Metabolism, Institute for Molecular and Cellular Regulation, Gunma University,...
推荐引用方式 GB/T 7714	Matsumura Y.,Ito R.,Yajima A.,等. Spatiotemporal dynamics of SETD5-containing NCoR–HDAC3 complex determines enhancer activation for adipogenesis[J],2021,12(1).
APA	Matsumura Y..,Ito R..,Yajima A..,Yamaguchi R..,Tanaka T..,...&Sakai J..(2021).Spatiotemporal dynamics of SETD5-containing NCoR–HDAC3 complex determines enhancer activation for adipogenesis.Nature Communications,12(1).
MLA	Matsumura Y.,et al."Spatiotemporal dynamics of SETD5-containing NCoR–HDAC3 complex determines enhancer activation for adipogenesis".Nature Communications 12.1(2021).