气候变化领域集成服务门户(CCPortal): High-volume sampler for size-selective sampling of bioaerosols including viruses

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DOI	10.1016/j.atmosenv.2021.118720
	High-volume sampler for size-selective sampling of bioaerosols including viruses
	Lim J.-H.; Nam S.H.; Kim J.; Kim N.H.; Park G.-S.; Maeng J.-S.; Yook S.-J.
发表日期	2021
ISSN	1352-2310
卷号	265
英文摘要	Owing to the recent global spread of the new coronavirus SARS-CoV-2, the development of technology to effectively detect viruses in crowded public places is urgently needed. In this study, a three-stage high-volume bioaerosol sampler was developed for the size-selective sampling of bioaerosols through the suction of air at a high flow rate of 1000 L/min. In stage 1, an omnidirectional inlet cyclone separator that can draw air from all directions was applied to collect bioaerosols larger than 10 μm in the collection fluid. In stage 2, an axial flow cyclone separator was used to collect bioaerosols sized between 2.5 and 10 μm in the collection fluid. In stage 3, bioaerosols smaller than 2.5 μm were collected on a filter and extracted in a solution through an elution process using a sodium phosphate buffer. To simulate the suspension of bioparticles including viruses that are attached to other particles in the atmosphere, the aerosol samples were prepared by coagulating aerosolized bacteriophages with Arizona test dust. Then, the coagulated particles were collected for 30 min using the developed bioaerosol sampler, and the samples collected in each stage were analyzed via polymerase chain reaction (PCR) method. The PCR analysis results confirmed that the high-volume bioaerosol sampler enables size-selective bioaerosol sampling even at a high airflow rate of 1000 L/min. The developed high-volume bioaerosol sampler will be useful in detecting viruses through PCR analysis because it can collect bioaerosols within a specific size range. © 2021 Elsevier Ltd
关键词	Bioaerosol Cyclone separator Filter High-volume sampler Size-selective sampler
语种	英语
scopus关键词	Air; Bacteriophages; Particle size analysis; Polymerase chain reaction; Sodium compounds; Suspensions (fluids); Bioaerosols; Filter; High volumes; High-flow rate; High-volume sampler; Public places; Reaction analysis; Selective sampling; Size-selective; Size-selective sampler; Aerosols; sodium dihydrogen phosphate; aerosol; air sampling; sampler; severe acute respiratory syndrome; virus; aerosol; airflow; Article; atmosphere; bacteriophage; bioaerosol; dust; elution; flow rate; nonhuman; particle size; polymerase chain reaction; pressure; simulation; suction; suspension; virus; Coronavirus; environmental samples (bacteriophages); SARS coronavirus
来源期刊	ATMOSPHERIC ENVIRONMENT
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/248216
作者单位	School of Mechanical Engineering, Hanyang University, Seoul, 04763, South Korea; Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Yuseong, Daejeon 34114, South Korea; Research Group of Food Processing, Korea Food Research Institute, Wanju, Jeonbuk 55365, South Korea
推荐引用方式 GB/T 7714	Lim J.-H.,Nam S.H.,Kim J.,et al. High-volume sampler for size-selective sampling of bioaerosols including viruses[J],2021,265.
APA	Lim J.-H..,Nam S.H..,Kim J..,Kim N.H..,Park G.-S..,...&Yook S.-J..(2021).High-volume sampler for size-selective sampling of bioaerosols including viruses.ATMOSPHERIC ENVIRONMENT,265.
MLA	Lim J.-H.,et al."High-volume sampler for size-selective sampling of bioaerosols including viruses".ATMOSPHERIC ENVIRONMENT 265(2021).