气候变化领域集成服务门户(CCPortal): Differential response of human lung epithelial cells to particulate matter in fresh and photochemically aged biomass-burning smoke

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DOI	10.1016/j.atmosenv.2021.118929
	Differential response of human lung epithelial cells to particulate matter in fresh and photochemically aged biomass-burning smoke
	Atwi K.; Wilson S.N.; Mondal A.; Edenfield R.C.; Symosko Crow K.M.; El Hajj O.; Perrie C.; Glenn C.K.; Easley C.A.; IV; Handa H.; Saleh R.
发表日期	2022
ISSN	1352-2310
英文摘要	The chemical composition of particulate matter (PM) in biomass-burning smoke evolves upon aging in the atmosphere. The effect of this evolution on the toxicity of biomass-burning PM is understudied. Here, we burned oak foliage, pine needles, and hickory twigs in an environmental chamber. We used UV radiation to initiate photochemical aging of the emissions leading to the production of secondary organic aerosol (SOA), quantified using online particle size distribution measurements, and an overall increase in the PM oxygenation and decrease in the relative abundance of aromatic and condensed aromatic structures, obtained using ultra-high-resolution electrospray ionization mass spectrometry. In vitro exposure of human lung epithelial cells to PM from hickory combustion led to the strongest reduction in metabolic activity, followed by pine and oak, which was associated with the heavy metal content of the PM from the three fuels, quantified using induction-coupled plasma mass spectrometry. Furthermore, exposure to the fresh PM led to more reduction in metabolic activity than the aged PM for all fuels, whereas the aged PM induced more cell death by apoptosis. The differential cellular response to the fresh and aged PM indicates that the increase in oxygenation and decrease in aromaticity associated with photochemical aging alters the toxicity mechanisms exhibited by the PM, with a possible role of decreasing the heavy metal content (gram-metals per gram-PM) due to SOA formation. Together, these findings highlight the complex effect of photochemical aging on biomass-burning PM toxicity and motivate further studies to elucidate the underlying differences in toxicity mechanisms between fresh and aged PM. © 2021 Elsevier Ltd
关键词	Apoptosis Atmospheric aging Mitochondrial dysfunction Organic aerosol Toxicity Wildland fires
语种	英语
scopus关键词	Aerosols; Aromatization; Biological organs; Biomass; Cell death; Electrospray ionization; Heavy metals; Mass spectrometry; Metabolism; Oxygenation; Particle size; Particle size analysis; Smoke; Atmospheric aging; Biomass burning smokes; Biomass-burning; Human lung; Lung epithelial cells; Mitochondrial dysfunction; Organic aerosol; Particulate Matter; Photochemicals; Wildland fire; Toxicity
来源期刊	ATMOSPHERIC ENVIRONMENT
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/248131
作者单位	Air Quality and Climate Research Laboratory, School of Environmental, Civil, Agricultural and Mechanical Engineering, University of Georgia, Athens, GA, United States; Handa Biomaterials Research Laboratory, School of Materials, Chemical and Biomedical Engineering, University of Georgia, Athens, GA, United States; Department of Environmental Health Science, College of Public Health, University of Georgia, Athens, GA, United States; Regenerative Bioscience Center, University of Georgia, Athens, GA, United States; Department of Pharmaceutical and Biomedical Sciences, University of Georgia, Athens, GA, United States
推荐引用方式 GB/T 7714	Atwi K.,Wilson S.N.,Mondal A.,et al. Differential response of human lung epithelial cells to particulate matter in fresh and photochemically aged biomass-burning smoke[J],2022.
APA	Atwi K..,Wilson S.N..,Mondal A..,Edenfield R.C..,Symosko Crow K.M..,...&Saleh R..(2022).Differential response of human lung epithelial cells to particulate matter in fresh and photochemically aged biomass-burning smoke.ATMOSPHERIC ENVIRONMENT.
MLA	Atwi K.,et al."Differential response of human lung epithelial cells to particulate matter in fresh and photochemically aged biomass-burning smoke".ATMOSPHERIC ENVIRONMENT (2022).