气候变化领域集成服务门户(CCPortal): Developmental clock and mechanism of de novo polarization of the mouse embryo

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DOI	10.1126/science.abd2703
	Developmental clock and mechanism of de novo polarization of the mouse embryo
	Zhu M.; Cornwall-Scoones J.; Wang P.; Handford C.E.; Na J.; Thomson M.; Zernicka-Goetz M.
发表日期	2020
ISSN	0036-8075
卷号	370 期号:6522
英文摘要	Embryo polarization is critical for mouse development; however, neither the regulatory clock nor the molecular trigger that it activates is known. Here, we show that the embryo polarization clock reflects the onset of zygotic genome activation, and we identify three factors required to trigger polarization. Advancing the timing of transcription factor AP-2 gamma (Tfap2c) and TEA domain transcription factor 4 (Tead4) expression in the presence of activated Ras homolog family member A (RhoA) induces precocious polarization as well as subsequent cell fate specification and morphogenesis. Tfap2c and Tead4 induce expression of actin regulators that control the recruitment of apical proteins on the membrane, whereas RhoA regulates their lateral mobility, allowing the emergence of the apical domain. Thus, Tfap2c, Tead4, and RhoA are regulators for the onset of polarization and cell fate segregation in the mouse. © 2020 American Association for the Advancement of Science. All rights reserved.
英文关键词	actin; ezrin; RhoA guanine nucleotide binding protein; TEA domain transcription factor 4; transcription factor; transcription factor AP 2 gamma; unclassified drug; actin; cytoskeleton protein; DNA binding protein; ezrin; muscle protein; RhoA guanine nucleotide binding protein; RhoA protein, mouse; Tead4 protein, mouse; Tfap2c protein, mouse; transcription factor; transcription factor AP 2; developmental biology; embryo; gene expression; genome; genomics; induced response; polarization; animal cell; animal experiment; Article; cell fate; cell membrane; controlled study; developmental stage; embryo; female; genome; male; molecular clock; morphogenesis; mouse; nonhuman; polarization; positive feedback; priority journal; protein expression; signal transduction; zygote; animal; biological rhythm; blastocyst; C57BL mouse; cell differentiation; cell polarity; cytology; embryo development; gene knockdown; genetics; metabolism; physiology; RNA interference; Actins; Animals; Biological Clocks; Blastocyst; Cell Differentiation; Cell Polarity; Cytoskeletal Proteins; DNA-Binding Proteins; Embryonic Development; Female; Gene Knockdown Techniques; Mice; Mice, Inbred C57BL; Muscle Proteins; rhoA GTP-Binding Protein; RNA Interference; Transcription Factor AP-2; Transcription Factors
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/246176
作者单位	Department of Physiology Development and Neuroscience, University of Cambridge, Downing Street, Cambridge, CB2 3DY, United Kingdom; Division of Biology and Biological Engineering, California Institute of Technology (Caltech), Pasadena, CA 91125, United States; Centre for Stem Cell Biology and Regenerative Medicine, School of Medicine, Tsinghua University, Beijing, 100084, China; Centre for Trophoblast Research, University of Cambridge, Cambridge, CB2 3EG, United Kingdom
推荐引用方式 GB/T 7714	Zhu M.,Cornwall-Scoones J.,Wang P.,et al. Developmental clock and mechanism of de novo polarization of the mouse embryo[J],2020,370(6522).
APA	Zhu M..,Cornwall-Scoones J..,Wang P..,Handford C.E..,Na J..,...&Zernicka-Goetz M..(2020).Developmental clock and mechanism of de novo polarization of the mouse embryo.Science,370(6522).
MLA	Zhu M.,et al."Developmental clock and mechanism of de novo polarization of the mouse embryo".Science 370.6522(2020).