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| DOI | 10.1126/science.1243259 |
| Single-cell metabolomics: Analytical and biological perspectives | |
| Zenobi R. | |
| 发表日期 | 2013 |
| ISSN | 0036-8075 |
| 卷号 | 342期号:6163 |
| 英文摘要 | There is currently much interest in broad molecular profiling of single cells; a cell's metabolome - its full complement of small-molecule metabolites - is a direct indicator of phenotypic diversity of single cells and a nearly immediate readout of how cells react to environmental influences. However, the metabolome is very difficult to measure at the single-cell level because of rapid metabolic dynamics, the structural diversity of the molecules, and the inability to amplify or tag small-molecule metabolites. Measurement techniques including mass spectrometry, capillary electrophoresis, and, to a lesser extent, optical spectroscopy and fluorescence detection have led to impressive advances in single-cell metabolomics. Even though none of these methodologies can currently measure the metabolome of a single cell completely, rapidly, and nondestructively, progress has been sufficient such that the field is witnessing a shift from feasibility studies to investigations that yield new biological insight. Particularly interesting fields of application are cancer biology, stem cell research, and monitoring of xenobiotics and drugs in tissue sections at the single-cell level. |
| 英文关键词 | dimeticone; ganglioside GM1; lactosylceramide; reduced nicotinamide adenine dinucleotide; reduced nicotinamide adenine dinucleotide phosphate; xenobiotic agent; nanomaterial; cancer; cytology; metabolism; molecular analysis; phenotype; xenobiotics; adherent cell; capillary electrophoresis; cell size; cell suspension; conformational transition; environmental stress; enzyme linked immunosorbent assay; epigenetics; flow cytometry; fluorescence; fluorescence resonance energy transfer; gene expression; genetic difference; human; major clinical study; mass spectrometry; metabolome; metabolomics; microfluidics; nonhuman; priority journal; review; Saccharomyces cerevisiae; single cell analysis; stem cell research; genetic procedures; genotype; metabolome; methodology; phenotype; spectroscopy; procedures; Biosensing Techniques; Electrophoresis, Capillary; Genotype; Mass Spectrometry; Metabolome; Metabolomics; Microfluidics; Nanostructures; Phenotype; Single-Cell Analysis; Spectrum Analysis; Biosensing Techniques; Electrophoresis, Capillary; Genotype; Mass Spectrometry; Metabolome; Metabolomics; Microfluidics; Nanostructures; Phenotype; Single-Cell Analysis; Spectrum Analysis |
| 语种 | 英语 |
| 来源期刊 | Science
 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/246143 |
| 作者单位 | Department of Chemistry and Applied Biosciences, ETH Zürich, CH-8093 Zürich, Switzerland |
| 推荐引用方式 GB/T 7714 | Zenobi R.. Single-cell metabolomics: Analytical and biological perspectives[J],2013,342(6163). |
| APA | Zenobi R..(2013).Single-cell metabolomics: Analytical and biological perspectives.Science,342(6163). |
| MLA | Zenobi R.."Single-cell metabolomics: Analytical and biological perspectives".Science 342.6163(2013). |
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