气候变化领域集成服务门户(CCPortal): A bacterial global regulator forms a prion

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DOI	10.1126/science.aai7776
	A bacterial global regulator forms a prion
	Yuan A.H.; Hochschild A.
发表日期	2017
ISSN	0036-8075
卷号	355 期号:6321
英文摘要	Prions are self-propagating protein aggregates that act as protein-based elements of inheritance in fungi. Although prevalent in eukaryotes, prions have not been identified in bacteria. Here we found that a bacterial protein, transcription terminator Rho of Clostridium botulinum (Cb-Rho), could form a prion.We identified a candidate prion-forming domain (cPrD) in Cb-Rho and showed that it conferred amyloidogenicity on Cb-Rho and could functionally replace the PrD of a yeast prion-forming protein. Furthermore, its cPrD enabled Cb-Rho to access alternative conformations in Escherichia coli - a soluble form that terminated transcription efficiently and an aggregated, self-propagating prion form that was functionally compromised. The prion form caused genome-wide changes in the transcriptome.Thus, Cb-Rho functions as a protein-based element of inheritance in bacteria, suggesting that the emergence of prions predates the evolutionary split between eukaryotes and bacteria.
英文关键词	bacterial protein; proteome; transcription termination factor Rho; transcriptome; amyloid; bacterial protein; prion; Rho factor; Saccharomyces cerevisiae protein; coliform bacterium; eukaryote; fungus; genome; heritability; physiology; prion disease; protein; yeast; Article; Clostridium botulinum; conformation; drug tolerance; Escherichia coli; immune evasion; infection; nonhuman; prion; priority journal; stop codon; yeast cell; amino acid sequence; chemistry; Clostridium botulinum; genetics; metabolism; molecular evolution; prion; protein domain; Bacteria (microorganisms); Clostridium botulinum; Escherichia coli; Eukaryota; Fungi; Amino Acid Sequence; Amyloid; Bacterial Proteins; Clostridium botulinum; Escherichia coli; Evolution, Molecular; Prions; Protein Domains; Rho Factor; Saccharomyces cerevisiae Proteins
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/246118
作者单位	Department of Microbiology and Immunobiology, Harvard Medical School, 4 Blackfan Circle, Boston, MA 02115, United States
推荐引用方式 GB/T 7714	Yuan A.H.,Hochschild A.. A bacterial global regulator forms a prion[J],2017,355(6321).
APA	Yuan A.H.,&Hochschild A..(2017).A bacterial global regulator forms a prion.Science,355(6321).
MLA	Yuan A.H.,et al."A bacterial global regulator forms a prion".Science 355.6321(2017).