气候变化领域集成服务门户(CCPortal): Maternal Huluwa dictates the embryonic body axis through b-catenin in vertebrates

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DOI	10.1126/science.aat1045
	Maternal Huluwa dictates the embryonic body axis through b-catenin in vertebrates
	Yan L.; Chen J.; Zhu X.; Sun J.; Wu X.; Shen W.; Zhang W.; Tao Q.; Meng A.
发表日期	2018
ISSN	0036-8075
卷号	362 期号:6417
英文摘要	The vertebrate body is formed by cell movements and shape change during embryogenesis. It remains undetermined which maternal signals govern the formation of the dorsal organizer and the body axis.We found that maternal depletion of huluwa, a previously unnamed gene, causes loss of the dorsal organizer, the head, and the body axis in zebrafish and Xenopus embryos. Huluwa protein is found on the plasma membrane of blastomeres in the future dorsal region in early zebrafish blastulas. Huluwa has strong dorsalizing and secondary axis-inducing activities, which require b-catenin but can function independent of Wnt ligand/receptor signaling. Mechanistically, Huluwa binds to and promotes the tankyrase-mediated degradation of Axin. Therefore, maternal Huluwa is an essential determinant of the dorsal organizer and body axis in vertebrate embryos. © 2018 American Association for the Advancement of Science.All right reserved.
英文关键词	amino acid; antisense oligodeoxynucleotide; axin; axin1 protein; axin2 protein; beta catenin; bone morphogenetic protein 4; cycloheximide; dickkopf 1 protein; dishevelled 2; enhanced green fluorescent protein; fzd3b protein; fzd5 protein; fzd7b protein; fzd8b protein; hemagglutinin; huluwa protein; low density lipoprotein receptor related protein 5; low density lipoprotein receptor related protein 6; morpholino oligonucleotide; protein; tankyrase; transcription factor Otx2; transcription factor Sox3; unclassified drug; Wnt protein; wnt11b protein; Wnt3a protein; Wnt8a protein; axin; beta catenin; Xenopus protein; zebrafish protein; adult; Article; blastomere; blastula; cell fate; cell membrane; chromosome 21; controlled study; cytoplasm; ectoderm; ectopic expression; embryo; embryo axis; embryo development; female; fertilization; gastrulation; gene insertion sequence; gene locus; gene overexpression; genetic transfection; human; human cell; immunoblotting; immunohistochemistry; in situ hybridization; in vitro study; internalization; male; mammal cell; mesodermal cell; molecular cloning; mouse embryo; neuroectoderm; nonhuman; oocyte; phenotype; poly(adenosine diphosphate) ribosylation; priority journal; protein degradation; protein localization; quantitative analysis; reverse transcription polymerase chain reaction; signal transduction; spemann gastrulation organizer; upregulation; vertebrate; wild type; Wnt signaling; Xenopus laevis; zebra fish; animal; body patterning; embryology; genetics; HEK293 cell line; maternal inheritance; metabolism; physiology; Animals; Axin Protein; beta Catenin; Body Patterning; Embryonic Development; HEK293 Cells; Humans; Maternal Inheritance; Proteolysis; Wnt Signaling Pathway; Xenopus laevis; Xenopus Proteins; Zebrafish; Zebrafish Proteins
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/246085
作者单位	Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China; MOE Key Laboratory of Protein Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China
推荐引用方式 GB/T 7714	Yan L.,Chen J.,Zhu X.,et al. Maternal Huluwa dictates the embryonic body axis through b-catenin in vertebrates[J],2018,362(6417).
APA	Yan L..,Chen J..,Zhu X..,Sun J..,Wu X..,...&Meng A..(2018).Maternal Huluwa dictates the embryonic body axis through b-catenin in vertebrates.Science,362(6417).
MLA	Yan L.,et al."Maternal Huluwa dictates the embryonic body axis through b-catenin in vertebrates".Science 362.6417(2018).