气候变化领域集成服务门户(CCPortal): Bug mapping and fitness testing of chemically synthesized chromosome X

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DOI	10.1126/science.aaf4706
	Bug mapping and fitness testing of chemically synthesized chromosome X
	Wu Y.; Li B.-Z.; Zhao M.; Mitchell L.A.; Xie Z.-X.; Lin Q.-H.; Wang X.; Xiao W.-H.; Wang Y.; Zhou X.; Liu H.; Li X.; Ding M.-Z.; Liu D.; Zhang L.; Liu B.-L.; Wu X.-L.; Li F.-F.; Dong X.-T.; Jia B.; Zhang W.-Z.; Jiang G.-Z.; Liu Y.; Bai X.; Song T.-Q.; Chen Y.; Zhou S.-J.; Zhu R.-Y.; Gao F.; Kuang Z.; Wang X.; Shen M.; Yang K.; Stracquadanio G.; Richardson S.M.; Lin Y.; Wang L.; Walker R.; Luo Y.; Ma P.-S.; Yang H.; Cai Y.; Dai J.; Bader J.S.; Boeke J.D.; Yuan Y.-J.
发表日期	2017
ISSN	0036-8075
卷号	355 期号:6329
英文摘要	Debugging a genome sequence is imperative for successfully building a synthetic genome. As part of the effort to build a designer eukaryotic genome, yeast synthetic chromosome X (synX), designed as 707,459 base pairs, was synthesized chemically. SynX exhibited good fitness under a wide variety of conditions. A highly efficient mapping strategy called pooled PCRTag mapping (PoPM), which can be generalized to any watermarked synthetic chromosome, was developed to identify genetic alterations that affect cell fitness ("bugs"). A series of bugs were corrected that included a large region bearing complex amplifications, a growth defect mapping to a recoded sequence in FIP1, and a loxPsym site affecting promoter function of ATP2. PoPM is a powerful tool for synthetic yeast genome debugging and an efficient strategy for phenotype-genotype mapping. © 2017, American Association for the Advancement of Science. All rights reserved.
英文关键词	ATP2 protein; DNA fragment; FIP1 protein; genomic DNA; green fluorescent protein; protein; red fluorescent protein; synthetic DNA; transfer RNA; unclassified drug; cells and cell components; chromosome; eukaryote; fitness; genetic analysis; growth rate; insect; polymerase chain reaction; yeast; 3' untranslated region; amplicon; Article; binding site; cell structure; chromosome rearrangement; controlled study; dilution; DNA extraction; DNA sequence; DNA synthesis; down regulation; endoreduplication; gene duplication; gene expression; gene locus; gene mapping; gene rearrangement; homologous recombination; karyotyping; nonhuman; nucleotide sequence; open reading frame; phenotype; plasmid; polymerase chain reaction; priority journal; progeny; promoter region; pulsed field gel electrophoresis; RNA analysis; RNA sequence; Saccharomyces cerevisiae; stop codon; whole genome sequencing; X chromosome; chemistry; chromosomal mapping; fungal genome; genetics; high throughput sequencing; procedures; reproductive fitness; Saccharomyces cerevisiae; synthetic biology; yeast artificial chromosome; Eukaryota; Base Sequence; Chromosomes, Artificial, Yeast; Gene Duplication; Genetic Fitness; Genome, Fungal; High-Throughput Nucleotide Sequencing; Physical Chromosome Mapping; Saccharomyces cerevisiae; Synthetic Biology
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/246070
作者单位	Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin, 300072, China; Institute for Systems Genetics, Department of Biochemistry and Molecular Pharmacology, New York University (NYU) Langone Medical Center, New York City, NY 10016, United States; High Throughput Biology Center and Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, United States; School of Computer Science and Electronic Engineering, University of Essex, Wivenhoe Park, Colchester, CO4 3SQ, United Kingdom; Key Laboratory of Industrial Biocatalysis (Ministry of Education), Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China; School of Biological Sciences, University of Edinburgh, Edinburgh, EH9 3BF, United Kingdom; B...
推荐引用方式 GB/T 7714	Wu Y.,Li B.-Z.,Zhao M.,et al. Bug mapping and fitness testing of chemically synthesized chromosome X[J],2017,355(6329).
APA	Wu Y..,Li B.-Z..,Zhao M..,Mitchell L.A..,Xie Z.-X..,...&Yuan Y.-J..(2017).Bug mapping and fitness testing of chemically synthesized chromosome X.Science,355(6329).
MLA	Wu Y.,et al."Bug mapping and fitness testing of chemically synthesized chromosome X".Science 355.6329(2017).