气候变化领域集成服务门户(CCPortal): lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription

CCPortal

DOI	10.1126/science.abf6582
	lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription
	Wu M.; Xu G.; Han C.; Luan P.-F.; Xing Y.-H.; Nan F.; Yang L.-Z.; Huang Y.; Yang Z.-H.; Shan L.; Yang L.; Liu J.; Chen L.-L.
发表日期	2021
ISSN	0036-8075
起始页码	547
结束页码	555
卷号	373 期号:6554
英文摘要	RNA polymerase I (Pol I) transcription takes place at the border of the fibrillar center (FC) and the dense fibrillar component (DFC) in the nucleolus. Here, we report that individual spherical FC/DFC units are coated by the DEAD-box RNA helicase DDX21 in human cells. The long noncoding RNA (lncRNA) SLERT binds to DDX21 RecA domains to promote DDX21 to adopt a closed conformation at a substoichiometric ratio through a molecular chaperone-like mechanism resulting in the formation of hypomultimerized and loose DDX21 clusters that coat DFCs, which is required for proper FC/DFC liquidity and Pol I processivity. Our results suggest that SLERT is an RNA regulator that controls the biophysical properties of FC/DFCs and thus ribosomal RNA production. © 2021 American Association for the Advancement of Science. All rights reserved.
英文关键词	cell; gene; gene expression; genetic analysis; molecular analysis; RNA; separation; chaperone; DDX21 protein, human; DEAD box protein; DNA directed RNA polymerase; long untranslated RNA; ribosome DNA; cell line; chemistry; genetic transcription; human; metabolism; nucleolus; protein conformation; protein domain; protein multimerization; Cell Line; Cell Nucleolus; DEAD-box RNA Helicases; DNA, Ribosomal; Humans; Molecular Chaperones; Protein Conformation; Protein Domains; Protein Multimerization; RNA Polymerase I; RNA, Long Noncoding; Transcription, Genetic
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/246067
作者单位	State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai, China; CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China; School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China
推荐引用方式 GB/T 7714	Wu M.,Xu G.,Han C.,et al. lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription[J],2021,373(6554).
APA	Wu M..,Xu G..,Han C..,Luan P.-F..,Xing Y.-H..,...&Chen L.-L..(2021).lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription.Science,373(6554).
MLA	Wu M.,et al."lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription".Science 373.6554(2021).