气候变化领域集成服务门户(CCPortal): Structure and dynamics of the yeast SWR1-nucleosome complex

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DOI	10.1126/science.aat7716
	Structure and dynamics of the yeast SWR1-nucleosome complex
	Willhoft O.; Ghoneim M.; Lin C.-L.; Chua E.Y.D.; Wilkinson M.; Chaban Y.; Ayala R.; McCormack E.A.; Ocloo L.; Rueda D.S.; Wigley D.B.
发表日期	2018
ISSN	0036-8075
卷号	362 期号:6411
英文摘要	The yeast SWR1 complex exchanges histone H2A in nucleosomes with Htz1 (H2A.Z in humans). The cryo-electron microscopy structure of the SWR1 complex bound to a nucleosome at 3.6-angstrom resolution reveals details of the intricate interactions between components of the SWR1 complex and its nucleosome substrate. Interactions between the Swr1 motor domains and the DNA wrap at superhelical location 2 distort the DNA, causing a bulge with concomitant translocation of the DNA by one base pair, coupled to conformational changes of the histone core. Furthermore, partial unwrapping of the DNA from the histone core takes place upon binding of nucleosomes to SWR1 complex.The unwrapping, as monitored by single-molecule data, is stabilized and has its dynamics altered by adenosine triphosphate binding but does not require hydrolysis. © 2018 American Association for the Advancement of Science. All Rights Reserved.
英文关键词	adenosine triphosphatase; adenosine triphosphate; Saccharomyces cerevisiae protein; Swr1 protein, S cerevisiae; chemistry; chromatin assembly and disassembly; cryoelectron microscopy; metabolism; nucleosome; protein domain; Saccharomyces cerevisiae; ultrastructure; Adenosine Triphosphatases; Adenosine Triphosphate; Chromatin Assembly and Disassembly; Cryoelectron Microscopy; Nucleosomes; Protein Domains; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/246036
作者单位	Section of Structural Biology, Department of Medicine, Imperial College London, London, SW7 2AZ, United Kingdom; Single Molecule Imaging Group, MRC London Institute of Medical Sciences, London, W12 0NN, United Kingdom; Molecular Virology, Department of Medicine, Imperial College London, London, W12 0NN, United Kingdom; Department of Molecular and Cell Biology, University of Leicester, Leicester, LE1 9HN, United Kingdom; Electron Bio-Imaging Centre (EBIC), Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom
推荐引用方式 GB/T 7714	Willhoft O.,Ghoneim M.,Lin C.-L.,et al. Structure and dynamics of the yeast SWR1-nucleosome complex[J],2018,362(6411).
APA	Willhoft O..,Ghoneim M..,Lin C.-L..,Chua E.Y.D..,Wilkinson M..,...&Wigley D.B..(2018).Structure and dynamics of the yeast SWR1-nucleosome complex.Science,362(6411).
MLA	Willhoft O.,et al."Structure and dynamics of the yeast SWR1-nucleosome complex".Science 362.6411(2018).