气候变化领域集成服务门户(CCPortal): Radical SAM-dependent carbon insertion into the nitrogenase M-cluster

CCPortal

DOI	10.1126/science.1224603
	Radical SAM-dependent carbon insertion into the nitrogenase M-cluster
	Wiig J.A.; Hu Y.; Lee C.C.; Ribbe M.W.
发表日期	2012
ISSN	0036-8075
起始页码	1672
结束页码	1675
卷号	337 期号:6102
英文摘要	The active site of nitrogenase, the M-cluster, is a metal-sulfur cluster containing a carbide at its core. Using radiolabeling experiments, we show that this carbide originates from the methyl group of S-adenosylmethionine (SAM) and that it is inserted into the M-cluster by the assembly protein NifB. Our SAM cleavage and deuterium substitution analyses suggest a similarity between the mechanism of carbon insertion by NifB and the proposed mechanism of RNA methylation by the radical SAM enzymes RlmN and Cfr, which involves methyl transfer from one SAM equivalent, followed by hydrogen atom abstraction from the methyl group by a 5′-deoxyadenosyl radical generated from a second SAM equivalent. This work is an initial step toward unraveling the importance of the interstitial carbide and providing insights into the nitrogenase mechanism.
英文关键词	5' deoxyadenosyl radical; carbon; deuterium; hydrogen; iron; nitrogenase; protein; protein NifB; radical; RNA; s adenosylmethionine; unclassified drug; biochemistry; carbon; deuterium; enzyme activity; experimental study; hydrogen; methylation; organic compound; protein; radical; reaction kinetics; RNA; article; Azotobacter vinelandii; carbon dynamics; carbon insertion; chemical bond; Clostridium pasteurianum; electron spin resonance; enzyme active site; enzyme mechanism; gene cluster; high performance liquid chromatography; isotope labeling; m cluster; methylation; nonhuman; priority journal; protein assembly; ultraviolet radiation
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/246027
作者单位	Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697-3900, United States
推荐引用方式 GB/T 7714	Wiig J.A.,Hu Y.,Lee C.C.,et al. Radical SAM-dependent carbon insertion into the nitrogenase M-cluster[J],2012,337(6102).
APA	Wiig J.A.,Hu Y.,Lee C.C.,&Ribbe M.W..(2012).Radical SAM-dependent carbon insertion into the nitrogenase M-cluster.Science,337(6102).
MLA	Wiig J.A.,et al."Radical SAM-dependent carbon insertion into the nitrogenase M-cluster".Science 337.6102(2012).