气候变化领域集成服务门户(CCPortal): Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq

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DOI	10.1126/science.aai8478
	Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq
	Venteicher A.S.; Tirosh I.; Hebert C.; Yizhak K.; Neftel C.; Filbin M.G.; Hovestadt V.; Escalante L.E.; Shaw M.L.; Rodman C.; Gillespie S.M.; Dionne D.; Luo C.C.; Ravichandran H.; Mylvaganam R.; Mount C.; Onozato M.L.; Nahed B.V.; Wakimoto H.; Curry W.T.; Iafrate A.J.; Rivera M.N.; Frosch M.P.; Golub T.R.; Brastianos P.K.; Getz G.; Patel A.P.; Monje M.; Cahill D.P.; Rozenblatt-Rosen O.; Louis D.N.; Bernstein B.E.; Regev A.; Suvà M.L.
发表日期	2017
ISSN	0036-8075
卷号	355 期号:6332
英文摘要	Tumor subclasses differ according to the genotypes and phenotypes of malignant cells as well as the composition of the tumor microenvironment (TME).We dissected these influences in isocitrate dehydrogenase (IDH)-mutant gliomas by combining 14,226 single-cell RNA sequencing (RNA-seq) profiles from 16 patient samples with bulk RNA-seq profiles from 165 patient samples. Differences in bulk profiles between IDH-mutant astrocytoma and oligodendroglioma can be primarily explained by distinct TME and signature genetic events, whereas both tumor types share similar developmental hierarchies and lineages of glial differentiation. As tumor grade increases, we find enhanced proliferation of malignant cells, larger pools of undifferentiated glioma cells, and an increase in macrophage over microglia expression programs in TME. Our work provides a unifying model for IDH-mutant gliomas and a general framework for dissecting the differences among human tumor subclasses.
英文关键词	isocitrate dehydrogenase; small cytoplasmic RNA; isocitrate dehydrogenase; cells and cell components; gene expression; genetic analysis; mutation; RNA; tumor; Article; astrocyte; astrocytoma; cancer cell; cancer genetics; cancer grading; cell differentiation; cell lineage; cell proliferation; cell subpopulation; clinical article; controlled study; gene expression; genotype; glia cell; glioma; glioma cell; human; human cell; human tissue; macrophage; microglia; neural stem cell; oligodendroglia; oligodendroglioma; phenotype; priority journal; RNA sequence; single cell analysis; stem cell; tumor microenvironment; brain tumor; cancer stem cell; cell lineage; classification; genetics; glioma; metabolism; pathology; principal component analysis; sequence analysis; single cell analysis; Brain Neoplasms; Cell Lineage; Glioma; Humans; Isocitrate Dehydrogenase; Macrophages; Microglia; Neoplasm Grading; Neoplastic Stem Cells; Principal Component Analysis; Sequence Analysis, RNA; Single-Cell Analysis; Tumor Microenvironment
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/245843
作者单位	Department of Pathology and Center for Cancer Research, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, United States; Broad Institute of Harvard, MIT, Cambridge, MA 02142, United States; Department of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, United States; Institute of Pathology, Faculty of Biology and Medicine, Centre Hospitalier Universitaire Vaudois, Lausanne, 1011, Switzerland; Department of Pediatric Oncology, Dana-Farber Cancer Institute, Children's Hospital Cancer Center, Boston, MA 02215, United States; Departments of Neurology, Neurosurgery, Pediatrics and Pathology, Stanford University School of Medicine, Stanford, CA 94305, United States; Howard Hughes Medical Institute, Chevy Chase, MD 20815, United States; Departments of Medicine and Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, United States; Koch Institute, Department of Biology, Massachusetts Institute of Technology, Camb...
推荐引用方式 GB/T 7714	Venteicher A.S.,Tirosh I.,Hebert C.,et al. Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq[J],2017,355(6332).
APA	Venteicher A.S..,Tirosh I..,Hebert C..,Yizhak K..,Neftel C..,...&Suvà M.L..(2017).Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq.Science,355(6332).
MLA	Venteicher A.S.,et al."Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq".Science 355.6332(2017).