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DOI	10.1126/science.abg2822
	Evolution of a virus-like architecture and packaging mechanism in a repurposed bacterial protein
	Tetter S.; Terasaka N.; Steinauer A.; Bingham R.J.; Clark S.; Scott A.J.P.; Patel N.; Leibundgut M.; Wroblewski E.; Ban N.; Stockley P.G.; Twarock R.; Hilvert D.
发表日期	2021
ISSN	0036-8075
起始页码	1220
结束页码	1224
卷号	372 期号:6547
英文摘要	Viruses are ubiquitous pathogens of global impact. Prompted by the hypothesis that their earliest progenitors recruited host proteins for virion formation, we have used stringent laboratory evolution to convert a bacterial enzyme that lacks affinity for nucleic acids into an artificial nucleocapsid that efficiently packages and protects multiple copies of its own encoding messenger RNA. Revealing remarkable convergence on the molecular hallmarks of natural viruses, the accompanying changes reorganized the protein building blocks into an interlaced 240-subunit icosahedral capsid that is impermeable to nucleases, and emergence of a robust RNA stem-loop packaging cassette ensured high encapsidation yields and specificity. In addition to evincing a plausible evolutionary pathway for primordial viruses, these findings highlight practical strategies for developing nonviral carriers for diverse vaccine and delivery applications. © 2021 American Association for the Advancement of Science. All rights reserved.
英文关键词	bacterial enzyme; bacterial protein; messenger RNA; nuclease; nucleic acid; 6,7-dimethyl-8-ribityllumazine synthase; bacterial protein; messenger RNA; multienzyme complex; ribonuclease; detection method; enzyme; enzyme activity; host-pathogen interaction; pathogen; protein; RNA; vaccine; virus; Article; gene cassette; gene mutation; genome; genomics; molecular evolution; nonhuman; polymerase chain reaction; RNA structure; virion; virus like agent; virus nucleocapsid; amino acid substitution; Aquifex; chemistry; cryoelectron microscopy; directed molecular evolution; enzymology; genetics; metabolism; protein domain; protein secondary structure; protein subunit; virus capsid; Bacteria (microorganisms); Amino Acid Substitution; Aquifex; Bacterial Proteins; Capsid; Cryoelectron Microscopy; Directed Molecular Evolution; Multienzyme Complexes; Nucleocapsid; Protein Domains; Protein Structure, Secondary; Protein Subunits; Ribonucleases; RNA, Messenger
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/245718
作者单位	Laboratory of Organic Chemistry, ETH Zurich, Zurich, 8093, Switzerland; Departments of Mathematics and Biology, University of York, York, YO10 5DD, United Kingdom; Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, LS2 9JT, United Kingdom; Institute of Molecular Biology and Biophysics, ETH Zurich, Zurich, 8093, Switzerland
推荐引用方式 GB/T 7714	Tetter S.,Terasaka N.,Steinauer A.,et al. Evolution of a virus-like architecture and packaging mechanism in a repurposed bacterial protein[J],2021,372(6547).
APA	Tetter S..,Terasaka N..,Steinauer A..,Bingham R.J..,Clark S..,...&Hilvert D..(2021).Evolution of a virus-like architecture and packaging mechanism in a repurposed bacterial protein.Science,372(6547).
MLA	Tetter S.,et al."Evolution of a virus-like architecture and packaging mechanism in a repurposed bacterial protein".Science 372.6547(2021).