气候变化领域集成服务门户(CCPortal): Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field

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DOI	10.1126/science.aat9077
	Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field
	South P.F.; Cavanagh A.P.; Liu H.W.; Ort D.R.
发表日期	2019
ISSN	0036-8075
卷号	363 期号:6422
英文摘要	Photorespiration is required in C3 plants to metabolize toxic glycolate formed when ribulose-1,5-bisphosphate carboxylase-oxygenase oxygenates rather than carboxylates ribulose-1,5-bisphosphate. Depending on growing temperatures, photorespiration can reduce yields by 20 to 50% in C3 crops. Inspired by earlier work, we installed into tobacco chloroplasts synthetic glycolate metabolic pathways that are thought to be more efficient than the native pathway. Flux through the synthetic pathways was maximized by inhibiting glycolate export from the chloroplast. The synthetic pathways tested improved photosynthetic quantum yield by 20%. Numerous homozygous transgenic lines increased biomass productivity by >40% in replicated field trials. These results show that engineering alternative glycolate metabolic pathways into crop chloroplasts while inhibiting glycolate export into the native pathway can drive increases in C3 crop yield under agricultural field conditions. y © The Authors, some rights reserved
英文关键词	catalase; glycolate dehydrogenase; glycolic acid; hydroxy acid oxidase A; malate synthase; oxidoreductase; unclassified drug; Article; bacterial gene; biomass; canopy; chloroplast; controlled study; crop production; Escherichia coli; gene expression; green alga; nonhuman; photorespiration; photosynthesis; priority journal; protein expression; RNA interference; season; tobacco
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/245566
作者单位	Global Change and Photosynthesis Research Unit, United States Department of Agriculture–Agricultural Research Service, Urbana, IL 61801, United States; Carl R. Woese Institute for Genomic Biology, University of Illinois, Urbana, IL 61801, United States; Department of Crop Sciences, University of Illinois, Urbana, IL 61801, United States; Department of Plant Biology, University of Illinois, Urbana, IL 61801, United States; Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, United States
推荐引用方式 GB/T 7714	South P.F.,Cavanagh A.P.,Liu H.W.,et al. Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field[J],2019,363(6422).
APA	South P.F.,Cavanagh A.P.,Liu H.W.,&Ort D.R..(2019).Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field.Science,363(6422).
MLA	South P.F.,et al."Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field".Science 363.6422(2019).