气候变化领域集成服务门户(CCPortal): Multiple nutrient stresses at intersecting Pacific Ocean biomes detected by protein biomarkers

CCPortal

DOI	10.1126/science.1256450
	Multiple nutrient stresses at intersecting Pacific Ocean biomes detected by protein biomarkers
	Saito M.A.; McIlvin M.R.; Moran D.M.; Goepfert T.J.; DiTullio G.R.; Post A.F.; Lamborg C.H.
发表日期	2014
ISSN	0036-8075
起始页码	1173
结束页码	1177
卷号	345 期号:6201
英文摘要	Marine primary productivity is strongly influenced by the scarcity of required nutrients, yet our understanding of these nutrient limitations is informed by experimental observations with sparse geographical coverage and methodological limitations. We developed a quantitative proteomic method to directly assess nutrient stress in high-light ecotypes of the abundant cyanobacterium Prochlorococcus across a meridional transect in the central Pacific Ocean. Multiple peptide biomarkers detected widespread and overlapping regions of nutritional stress for nitrogen and phosphorus in the North Pacific Subtropical Gyre and iron in the equatorial Pacific. Quantitative protein analyses demonstrated simultaneous stress for these nutrients at biome interfaces. This application of proteomic biomarkers to diagnose ocean metabolism demonstrated Prochlorococcus actively and simultaneously deploying multiple biochemical strategies for low-nutrient conditions in the oceans. © 2014, American Association for the Advancement of Science. All rights reserved.
英文关键词	alkaline phosphatase; ammonia; biological marker; chlorophyll a; ferredoxin; flavodoxin; iron; nitrogen; phosphorus; superoxide dismutase; urea; biological marker; cobalt; nitrogen; phosphorus; protein; sea water; abundance; biomarker; biome; cyanobacterium; ecotype; gyre; iron; nitrogen; nutrient availability; phosphorus; primary production; protein; proteomics; quantitative analysis; stress field; transect; amino acid sequence; Article; bacterial genome; biome; controlled study; ecotype; hydrography; iron metabolism; marine environment; mass spectrometry; metagenome; microbial metabolism; nitrogen metabolism; nonhuman; nutrient limitation; nutrient stress; nutritional status; oxidative stress; Pacific Ocean; phosphate metabolism; photosynthesis; phytoplankton; population abundance; priority journal; Prochlorococcus; protein analysis; proteomics; quantitative analysis; stoichiometry; article; metabolism; methodology; microbiology; Pacific Ocean; physiological stress; Prochlorococcus; Pacific Ocean; Prochlorococcus; Biological Markers; Cobalt; Iron; Nitrogen; Pacific Ocean; Phosphorus; Prochlorococcus; Proteins; Proteomics; Seawater; Stress, Physiological
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/245320
作者单位	Marine Chemistry and Geochemistry Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, United States; College of Charleston, Charleston, SC, United States; Marine Biological Laboratory, Woods Hole, MA 02543, United States
推荐引用方式 GB/T 7714	Saito M.A.,McIlvin M.R.,Moran D.M.,et al. Multiple nutrient stresses at intersecting Pacific Ocean biomes detected by protein biomarkers[J],2014,345(6201).
APA	Saito M.A..,McIlvin M.R..,Moran D.M..,Goepfert T.J..,DiTullio G.R..,...&Lamborg C.H..(2014).Multiple nutrient stresses at intersecting Pacific Ocean biomes detected by protein biomarkers.Science,345(6201).
MLA	Saito M.A.,et al."Multiple nutrient stresses at intersecting Pacific Ocean biomes detected by protein biomarkers".Science 345.6201(2014).