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DOI	10.1126/science.abe6821
	Maturation of the matrix and viral membrane of HIV-1
	Qu K.; Ke Z.; Zila V.; Anders-Össwein M.; Glass B.; Mücksch F.; Müller R.; Schultz C.; Müller B.; Kräusslich H.-G.; Briggs J.A.G.
发表日期	2021
ISSN	0036-8075
起始页码	700
结束页码	704
卷号	373 期号:6555
英文摘要	Gag, the primary structural protein of HIV-1, is recruited to the plasma membrane for virus assembly by its matrix (MA) domain. Gag is subsequently cleaved into its component domains, causing structural maturation to repurpose the virion for cell entry. We determined the structure and arrangement of MA within immature and mature HIV-1 through cryo-electron tomography. We found that MA rearranges between two different hexameric lattices upon maturation. In mature HIV-1, a lipid extends out of the membrane to bind with a pocket in MA. Our data suggest that proteolytic maturation of HIV-1 not only assembles the viral capsid surrounding the genome but also repurposes the membrane-bound MA lattice for an entry or postentry function and results in the partial removal of up to 2500 lipids from the viral membrane. © 2021 American Association for the Advancement of Science. All rights reserved.
英文关键词	cell; genome; lipid; maturation; protein; virus; article; electron tomography; Human immunodeficiency virus 1; nonhuman; virus capsid; virus envelope; chemistry; Human immunodeficiency virus 1; lipid bilayer; metabolism; molecular model; physiology; protein conformation; protein domain; protein secondary structure; ultrastructure; virion; virus assembly; virus envelope; Gag protein; Human immunodeficiency virus antigen; membrane lipid; p17 protein, Human Immunodeficiency Virus Type 1; virus envelope protein; Capsid; Electron Microscope Tomography; env Gene Products, Human Immunodeficiency Virus; gag Gene Products, Human Immunodeficiency Virus; HIV Antigens; HIV-1; Lipid Bilayers; Membrane Lipids; Models, Molecular; Protein Conformation; Protein Domains; Protein Structure, Secondary; Viral Envelope; Virion; Virus Assembly
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/245152
作者单位	Structural Studies Division, Medical Research Council Laboratory of Molecular Biology, Cambridge, CB2 0QH, United Kingdom; Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, 69117, Germany; Molecular Medicine Partnership Unit, European Molecular Biology Laboratory, Universitätsklinikum Heidelberg, Heidelberg, 69117, Germany; Department of Infectious Diseases, Virology, Universitätsklinikum Heidelberg, Heidelberg, 69120, Germany; Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, 69117, Germany; Department of Chemical Physiology and Biochemistry, Oregon Health and Science University, Portland, OR 97239, United States; German Center for Infection Research, Heidelberg, Germany; Laboratory of Retrovirology, The Rockefeller University, New York, NY 10065, United States
推荐引用方式 GB/T 7714	Qu K.,Ke Z.,Zila V.,et al. Maturation of the matrix and viral membrane of HIV-1[J],2021,373(6555).
APA	Qu K..,Ke Z..,Zila V..,Anders-Össwein M..,Glass B..,...&Briggs J.A.G..(2021).Maturation of the matrix and viral membrane of HIV-1.Science,373(6555).
MLA	Qu K.,et al."Maturation of the matrix and viral membrane of HIV-1".Science 373.6555(2021).